African swine fever virus IAP-like protein induces the activation of nuclear factor kappa B

Journal of Virology
Clara I RodríguezYolanda Revilla

Abstract

African swine fever virus (ASFV) encodes a homologue of the inhibitor of apoptosis (IAP) that promotes cell survival by controlling the activity of caspase-3. Here we show that ASFV IAP is also able to activate the transcription factor NF-kappaB. Thus, transient transfection of the viral IAP increases the activity of an NF-kappaB reporter gene in a dose-responsive manner in Jurkat cells. Similarly, stably transfected cells expressing ASFV IAP have elevated basal levels of c-rel, an NF-kappaB-dependent gene. NF-kappaB complexes in the nucleus were increased in A224L-expressing cells compared with control cells upon stimulation with phorbol myristate acetate (PMA) plus ionomycin. This resulted in greater NF-kappaB-dependent promoter activity in ASFV IAP-expressing than in control cells, both in basal conditions and after PMA plus ionophore stimulation. The elevated NF-kappaB activity seems to be the consequence of higher IkappaB kinase (IKK) basal activity in these cells. The NF-kappaB-inducing activity of ASFV IAP was abrogated by an IKK-2 dominant negative mutant and enhanced by expression of tumor necrosis factor receptor-associated factor 2.

References

May 1, 1992·Immunology Today·L A Tartaglia, D V Goeddel
Jan 1, 1985·Current Topics in Microbiology and Immunology·E Viñuela
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·D C SchererD W Ballard
Nov 15, 1995·Genes & Development·I M VermaS Miyamoto
Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Feb 1, 1997·Current Biology : CB·V R Baichwal, P A Baeuerle
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z L ChuD W Ballard
May 15, 1998·The Journal of Biological Chemistry·Y RevillaM Fresno
May 23, 1998·Annual Review of Immunology·S GhoshE B Kopp
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M G SannaR J Ulevitch
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·F S LeeT Maniatis
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Feb 17, 1999·Genes & Development·Q L Deveraux, J C Reed
Jun 8, 1999·Annual Review of Immunology·D WallachM P Boldin
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q HuangJ C Reed
Mar 25, 2000·Molecular and Cellular Biology·C ChenC Gélinas
May 16, 2000·The Journal of Biological Chemistry·R Hofer-WarbinekR de Martin

❮ Previous
Next ❯

Citations

Nov 8, 2008·Nature Reviews. Immunology·Andrew G Bowie, Leonie Unterholzner
Mar 9, 2011·Nature Reviews. Microbiology·Masmudur M Rahman, Grant McFadden
Jun 15, 2004·Journal of Virology·Aitor G GranjaYolanda Revilla
Jun 3, 2008·PLoS Pathogens·Lorenzo GalluzziGuido Kroemer
Dec 25, 2012·Virus Research·Covadonga AlonsoRaquel Muñoz-Moreno
Nov 10, 2012·Virus Research·Sandra BlomeMartin Beer
Sep 4, 2010·Mitochondrion·Akane Ohta, Yukihiro Nishiyama
Feb 19, 2008·Biochemical and Biophysical Research Communications·Lan WangDalong Ma
Sep 18, 2007·Biochemical Pharmacology·Leonie Unterholzner, Andrew G Bowie
Feb 6, 2015·Seminars in Cell & Developmental Biology·Rollie J Clem
May 11, 2017·Viruses·Inmaculada Galindo, Covadonga Alonso
Oct 11, 2017·Vaccines·Marisa AriasUNKNOWN Sanchez-Vizcaino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis