African swine fever virus protein pE296R is a DNA repair apurinic/apyrimidinic endonuclease required for virus growth in swine macrophages.

Journal of Virology
Modesto Redrejo-RodríguezJ Salas

Abstract

We show here that the African swine fever virus (ASFV) protein pE296R, predicted to be a class II apurinic/apyrimidinic (AP) endonuclease, possesses endonucleolytic activity specific for AP sites. Biochemical characterization of the purified recombinant enzyme indicated that the K(m) and catalytic efficiency values for the endonucleolytic reaction are in the range of those reported for Escherichia coli endonuclease IV (endo IV) and human Ape1. In addition to endonuclease activity, the ASFV enzyme has a proofreading 3'-->5' exonuclease activity that is considerably more efficient in the elimination of a mismatch than in that of a correctly paired base. The three-dimensional structure predicted for the pE296R protein underscores the structural similarities between endo IV and the viral protein, supporting a common mechanism for the cleavage reaction. During infection, the protein is expressed at early times and accumulates at later times. The early enzyme is localized in the nucleus and the cytoplasm, while the late protein is found only in the cytoplasm. ASFV carries two other proteins, DNA polymerase X and ligase, that, together with the viral AP endonuclease, could act as a viral base excision repair system to protect the viru...Continue Reading

References

Sep 1, 1976·The Journal of General Virology·L EnjuanesE Viñuela
May 1, 1990·Mutation Research·T Lindahl
Apr 1, 1991·Experimental Cell Research·C Terai, D A Carson
May 5, 1991·Journal of Molecular Biology·F W Studier
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1985·Journal of Virology·A L CarrascosaE Viñuela
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·P C HanawaltJ L Couch
Jan 1, 1967·Archiv für die gesamte Virusforschung·J D AlmeidaW Plowright
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F Wohlrab, B Francke
Mar 1, 1984·Virology·V LeyA Talavera
Jan 1, 1982·Journal of Virological Methods·A L CarrascosaE Viñuela
Jun 1, 1995·Journal of Biotechnology·R GarcíaJ F Rodríguez
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T NunoshibaB Demple
Jun 1, 1993·Current Opinion in Cell Biology·D E BarnesB Sedgwick
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Karlin, S F Altschul
Aug 1, 1993·Virology·S S Broyles
Aug 1, 1993·The Journal of General Virology·R J YáñezE Viñuela
Mar 1, 1993·Virology·R J Yáñez, E Viñuela
Dec 1, 1996·Protein Science : a Publication of the Protein Society·G S PrasadC D Stout
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·R A BennettB Demple
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·J A HoughtonD M Tillman
Nov 5, 1997·The Journal of Biological Chemistry·A M ChagovetzB D Preston
Jan 10, 1998·The Journal of Biological Chemistry·M OliverosL Blanco
Apr 16, 1998·The Journal of Biological Chemistry·D PrangishviliW Zillig
Jul 31, 1998·Experimental Cell Research·N KudoM Yoshida

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Citations

Jul 15, 2005·Journal of Virology·Yuanzheng ZhangJames L Van Etten
Jul 24, 2008·Avian Diseases·Li-chan ZhaoTao Sun
Nov 13, 2012·Virus Research·Linda K DixonChris Upton
Oct 9, 2012·Virus Research·Patricia de LeónAngel L Carrascosa
Nov 5, 2013·Virus Research·Modesto Redrejo-Rodríguez, María L Salas
Mar 21, 2007·Neuroscience·T DavidsenT Tønjum
Oct 29, 2008·Journal of Molecular Biology·Benedetta A Sampoli BenítezTamar Schlick
Jan 8, 2013·Virus Research·Christopher L Netherton, Thomas E Wileman
Oct 13, 2012·Virus Research·María L Salas, Germán Andrés
Sep 6, 2011·Virus Research·Li-Bo HeQi-Ya Zhang
Sep 7, 2018·Journal of Virology·Alí AlejoGermán Andrés
Mar 21, 2020·Cell Discovery·Yiqing ChenJianhua Gan
Mar 18, 2021·Cell Discovery·Yiqing ChenJianhua Gan
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiwon ChoiSoon B Hwang

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