African swine fever virus structural protein p72 contains a conformational neutralizing epitope

Virology
M V BorcaD L Rock

Abstract

We have previously described a monoclonal antibody (mAb 135D4) to an unidentified 70- to 72-kDa African swine fever virus (ASFV) protein that exhibited high levels of neutralizing activity against various virulent ASFV isolates. Here, we identify the reactive ASFV protein as the major virus structural protein p72. In vitro-translated products of the p72 protein gene were specifically immunoprecipitated by mAb 135D4. Immunoprecipitation of a nested set of truncated p72 in vitro translation products defined the region between amino acid residues 400 and 404 as necessary for mAb 135D4 reactivity. Five partially overlapping peptides (15mers) covering residues 388-446 failed to react with mAb 135D4, suggesting the conformational dependence of the epitope. Supporting this interpretation, larger in vitro translation products representing residues 56-282, 159-361, 360-508, and 507-646 also failed to react with mAb 135D4. Consistent with its involvement in virus neutralization, immunoelectromicroscopy, using a rabbit antiserum against mAb 135D4-purified p72, located the protein on the surface of unenveloped virus particles.

Citations

Mar 5, 2016·Veterinary Microbiology·Hovakim Zakaryan, Yolanda Revilla
Nov 20, 2012·Virus Research·José M EscribanoCovadonga Alonso
Jun 23, 2004·Veterinary Immunology and Immunopathology·Linda K DixonFuquan Zhang
Oct 11, 2017·Vaccines·Marisa AriasUNKNOWN Sanchez-Vizcaino
Jan 11, 2019·The Journal of General Virology·Galina BurmakinaDaniel L Rock
Jul 7, 2018·Journal of Veterinary Research·Ning JiaJie Zhang
May 24, 2020·Vaccines·Lynnette C GoatleyChristopher L Netherton
Jan 13, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mallory E HeimermanRaymond R Rowland
Apr 2, 2020·Journal of Veterinary Science·Dongwan YooHan Sang Yoo
Jun 28, 2019·Vaccines·Natasha N Gaudreault, Juergen A Richt
Mar 14, 1998·Journal of Virology·I RouillerT Wileman
Feb 5, 2019·Veterinary Immunology and Immunopathology·Daniel Pérez-NúñezYolanda Revilla
Jun 3, 2021·Viruses·Daniel L Rock

❮ Previous
Next ❯