Afterhyperpolarization induced by the activation of nicotinic acetylcholine receptors in pelvic ganglion neurons of male rats

Neuroscience Letters
Kyu-Sang ParkIn Deok Kong

Abstract

The electrophysiological mechanism underlying afterhyperpolarization induced by the activation of the nicotinic acetylcholine receptor (nAChR) in male rat major pelvic ganglion neurons (MPG) was investigated using a gramicidin-perforated patch clamp and microscopic fluorescence measurement system. Acetylcholine (ACh) induced fast depolarization through the activation of nAChR, followed by a sustained hyperpolarization after the removal of ACh in a dose-dependent manner (10 microM to 1mM). ACh increased both intracellular Ca(2+) ([Ca(2+)](i)) and Na(+) concentrations ([Na(+)](i)) in MPG neurons. The recovery of [Na(+)](i) after the removal of ACh was markedly delayed by ouabain (100 microM), an inhibitor of Na(+)/K(+) ATPase. Pretreatment with ouabain blocked ACh-induced hyperpolarization by 67.2+/-5.4% (n=7). ACh-induced hyperpolarization was partially attenuated by either the chelation of [Ca(2+)](i) with BAPTA/AM (20 microM) or the blockade of small-conductance Ca(2+)-activated K(+) channels by apamin (500 nM). Taken together, the activation of nAChR increases [Na(+)](i) and [Ca(2+)](i), which activates Na(+)/K(+) ATPase and Ca(2+)-activated K(+) channels, respectively. Consequently, hyperpolarization occurs after the activat...Continue Reading

References

Mar 1, 1988·British Journal of Pharmacology·M Feletou, P M Vanhoutte
Dec 1, 1987·Journal of the Autonomic Nervous System·V I Skok
Mar 1, 1983·Neuroscience·E KatoT Narahashi
Sep 21, 2000·The New England Journal of Medicine·S VerninoV A Lennon
Jun 11, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chris T BondJohn P Adelman
Nov 13, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ji Eun LeeSung Joon Kim
Apr 26, 2006·Pflügers Archiv : European journal of physiology·Igor I KrivoiFrederic Mandel

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Citations

Jun 3, 2016·Frontiers in Physiology·Vladimir V Matchkov, Igor I Krivoi
Jul 30, 2020·Molecular Biology of the Cell·Brianna R KingTrisha N Davis

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