Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30(T-)).

Journal of Veterinary Science
Seong In LimChang Hee Kweon

Abstract

Bovine leukemia virus (BLV) envelope glycoprotein (gp51/ gp30(T-)), consisting of BLV gp51 and BLV gp30 that lacked its C-terminal transmembrane domain, was expressed in insect cells under the control of the baculovirus polyhedron promoter. Recombinant BLV gp51/gp30(T-) secreted from insect cells was determined by immunofluorescence, enzyme-linked immunosorbent and western blot assays using a BLV-specific monoclonal antibody and BLV-positive bovine antibodies. An agar gel immunodiffusion (AGID) test using gp51/gp30(T-) as the antigen for the detection of BLV antibodies in serum was developed and compared to traditional AGID, which uses wild type BLV antigen derived from fetal lamb kidney cells. AGID with the recombinant BLV gp51/gp30(T-) was relatively more sensitive than traditional AGID. When the two methods were tested with bovine sera from the field, the recombinant BLV gp51/gp30(T-) and traditional antigen had a relative sensitivity of 69.8% and 67.4%, respectively, and a relative specificity of 93.3% and 92.3%. These results indicated that the recombinant BLV gp51/gp30(T-) is an effective alternative antigen for the diagnosis of BLV infection in cattle.

References

Dec 1, 1977·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J M Miller, M J Van Der Maaten
Feb 1, 1992·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·A Ballagi-PordányS Belák
Feb 1, 1992·Zentralblatt für Veterinärmedizin. Reihe A·Y SanadaH Nagahata
Jan 22, 1998·Veterinary Immunology and Immunopathology·A DoménechE Gómez-Lucía
Feb 13, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Christopher J KuckleburgJane Christopher-Hennings
Mar 26, 2003·The Journal of Veterinary Medical Science·Tatsufumi UsuiMisao Onuma
Sep 24, 2004·Journal of Virological Methods·D BeierO Marquardt

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Citations

Apr 11, 2012·Archives of Virology·Davor BalićJosip Madić
Nov 4, 2017·Virology Journal·Meripet PolatYoko Aida
Jul 28, 2019·The Journal of General Virology·Monireh KazemimaneshKayhan Azadmanesh

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Datasets Mentioned

BETA
AY995174

Methods Mentioned

BETA
enzyme-linked immunosorbent assays
ELISA
protein assay
PCR

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