Agar medium for use in susceptibility testing of bacteria from human periodontal pockets.

Antimicrobial Agents and Chemotherapy
C B WalkerS S Socransky

Abstract

An agar medium (medium V) was formulated to determine the minimal inhibitory concentrations (MICs) of antimicrobial agents for bacteria encountered in human periodontal pockets. The medium contained (per liter) Trypticase, 15 g; yeast extract, 5 g; sodium chloride, 5 g; glucose, 2 g; sodium pyruvate, 2 g; sodium formate, 1 g; sodium fumarate, 1.5 g; sodium succinate, 0.1 g; Tween 80, 0.25 ml; agar, 15 g; hemin, 5 mg; and menadione, 0.5 mg. The growth of 50 oral strains was compared on this and six other media which included: Wilkins-Chalgren agar, Schaedler agar, Brucella agar, Trypticase-soy blood agar, and Schaedler and Brucella agars supplemented with whole blood. Growth, for most strains, was greatest on medium V. Medium V was also compared with Wilkins-Chalgren agar, using the same oral strains, to determine the MICs of the following antibiotics: penicillin, tetracycline, chloramphenicol, clindamycin, and erythromycin. The MICs of these antibiotics were essentially the same on both media when growth was quantitatively similar.

References

Jan 1, 1979·Journal of Periodontal Research·M A ListgartenR Parodi
Dec 1, 1976·Antimicrobial Agents and Chemotherapy·T D Wilkins, S Chalgren
Nov 1, 1975·Applied Microbiology·T D Wilkins, C B Walker
Oct 1, 1971·Journal of Bacteriology·M LevA F Milford
Jul 1, 1965·The Journal of Experimental Medicine·R W SCHAEDLERR COSTELLO

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Citations

Jul 10, 2001·Journal of Clinical Periodontology·J G CatonC Walker
Aug 1, 1981·Antimicrobial Agents and Chemotherapy·V L SutterY Y Kwok
Mar 1, 1983·Antimicrobial Agents and Chemotherapy·V L SutterA T Ghoneim
Sep 1, 1983·Antimicrobial Agents and Chemotherapy·P J BakerR T Evans
Nov 1, 1986·Antimicrobial Agents and Chemotherapy·J L RummensH W Van Landuyt
Dec 1, 1987·Antimicrobial Agents and Chemotherapy·P W CaufieldN K Childers
Jan 5, 2008·Oral Microbiology and Immunology·M Al-HaroniP Cash
Apr 1, 1981·Scandinavian Journal of Dental Research·I Olsen, S S Socransky
Apr 1, 1981·Journal of Clinical Periodontology·J M GordonS S Socransky
Nov 1, 1988·Journal of Periodontology·R H Stevens, B F Hammond
Nov 1, 1990·Journal of Periodontology·C Walker, J Gordon
Jul 1, 1982·Journal of Endodontics·A C TannerS S Socransky
Sep 1, 1987·Oral Microbiology and Immunology·C B WalkerC J King
Jan 1, 1982·Applied and Environmental Microbiology·S M HuijghebaertH J Eyssen
Apr 1, 1983·Journal of Clinical Microbiology·E J GoldsteinJ R Berger

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