Agarose-dextran gels as synthetic analogs of glomerular basement membrane: water permeability

Biophysical Journal
Jeffrey A White, William M Deen

Abstract

Novel agarose-dextran hydrogels were synthesized and their suitability as experimental models of glomerular basement membrane was examined by measuring their Darcy (hydraulic) permeabilities (kappa). Immobilization of large dextran molecules in agarose was achieved by electron beam irradiation. Composite gels were made with agarose volume fractions (phi(a)) of 0.04 or 0.08 and dextran volume fractions (phi(d)) ranging from 0 to 0.02 (fiber volume/gel volume), using either of two dextran molecular weights (500 or 2000). At either agarose concentration and for either size of dextran, kappa decreased markedly as the amount of dextran was increased. Statistically significant deviations from the value of kappa for pure agarose were obtained for remarkably small volume fractions of dextran: phi(d) > or = 0.0003 for phi(a) = 0.04 and phi(d) > or = 0.001 for phi(a) = 0.08. The Darcy permeabilities were much more sensitive to phi(d) than to phi(a), and were as much as 26 times smaller than those of pure agarose. Although phi(d) was an important variable, dextran molecular weight was not. The effects of dextran addition on kappa were described fairly well using simple structural idealizations. At high agarose concentrations, the dextran ...Continue Reading

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Citations

Nov 13, 2009·Tissue Engineering. Part C, Methods·Yu-Chieh ChiuEric M Brey
Feb 22, 2012·Sensors·Brooke BeierPedro Irazoqui
Jul 25, 2009·Journal of the Mechanical Behavior of Biomedical Materials·Triantafyllos StylianopoulosVictor H Barocas
Sep 22, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Tsuneo TataraKimihiko Fujita
Aug 19, 2009·Biophysical Journal·Nadeen O ChahineGerard A Ateshian
Nov 2, 2004·Biophysical Journal·Kimberly B Kosto, William M Deen
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Aug 25, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Christophe SchatzThierry Delair

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