Age affects myosin relaxation states in skeletal muscle fibers of female but not male mice

PloS One
Lien A PhungDawn A Lowe

Abstract

The recent discovery that myosin has two distinct states in relaxed muscle-disordered relaxed (DRX) and super-relaxed (SRX)-provides another factor to consider in our fundamental understanding of the aging mechanism in skeletal muscle, since myosin is thought to be a potential contributor to dynapenia (age-associated loss of muscle strength independent of atrophy). The primary goal of this study was to determine the effects of age on DRX and SRX states and to examine their sex specificity. We have used quantitative fluorescence microscopy of the fluorescent nucleotide analog 2'/3'-O-(N-methylanthraniloyl) ATP (mantATP) to measure single-nucleotide turnover kinetics of myosin in skinned skeletal muscle fibers under relaxing conditions. We examined changes in DRX and SRX in response to the natural aging process by measuring the turnover of mantATP in skinned fibers isolated from psoas muscle of adult young (3-4 months old) and aged (26-28 months old) C57BL/6 female and male mice. Fluorescence decays were fitted to a multi-exponential decay function to determine both the time constants and mole fractions of fast and slow turnover populations, and significance was analyzed by a t-test. We found that in females, both the DRX and SRX...Continue Reading

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Citations

Jul 16, 2020·Expert Review of Hematology·Livio PaganoAlessandro Busca
Oct 1, 2020·American Journal of Physiology. Cell Physiology·Lien A PhungDavid D Thomas
Feb 11, 2021·ELife·Suman Nag, Darshan V Trivedi
Jun 12, 2021·International Journal of Endocrinology·Amira Mohammed Ali, Hiroshi Kunugi

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Methods Mentioned

BETA
nucleotide chase
X-ray
nucleotide exchange
dissection
Fluorescence
fluorescence imaging
fluorescence microscopy
biopsies
hormone replacement therapy

Software Mentioned

Origin
ImageJ
SPSS

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