Age and diet act through distinct isoforms of the class II transactivator gene in mouse intestinal epithelium

Gastroenterology
Ian R SandersonDemetra S Stamm

Abstract

Normal weaning induces class II major histocompatibility complex (Ia) and invariant chain (Ii) expression in the mouse intestinal epithelium. Because the class II transactivator protein (CIITA) induces Ia and Ii in most cell types, we hypothesized that diet-induced expression of these genes was through CIITA. Mouse litters were split and weaned onto an elemental diet or a normal (complex) chow diet. On days 24, 31, and 45, epithelial cells were isolated from small intestine with EDTA, and the RNA was extracted from both wild-type and interferon (IFN)-gamma receptor knockout mice. Messenger RNA (mRNA) was measured by Northern blotting, RNase protection assay, and real-time polymerase chain reaction and Ia localized by immunohistochemistry. By day 31, CIITA mRNA was induced in the intestinal epithelium of normally weaned wild-type mice, and this mirrored the expression of Ii chain mRNA. Mice weaned onto an elemental diet did not exhibit Ii mRNA or increased CIITA mRNA in the intestinal epithelium by day 31, but low levels of Ii mRNA were detectable by day 45. Of the 3 isoforms of CIITA, weaning onto a complex diet induced only CIITA IV by day 31. Mice deficient in the IFN-gamma receptor expressed Ia in the epithelium and they als...Continue Reading

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Citations

Jul 18, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ian R Sanderson, W Allan Walker
Mar 10, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R J CuthbertM A Hull
Jun 28, 2005·JPEN. Journal of Parenteral and Enteral Nutrition·Ian R Sanderson, Nicholas M Croft
Jul 18, 2012·EMBO Reports·Johanna Pott, Mathias Hornef
Oct 6, 2017·Frontiers in Immunology·Andrea C Lei-LestonKevin J Maloy
Oct 24, 2007·The Journal of Nutrition·Ian R Sanderson
Oct 3, 2018·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Sep 24, 2020·Immunology·Cornelia HeubergerKevin Joseph Maloy

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