Age and gender specific stimulation of creatine kinase specific activity by gonadal steroids in human bone-derived cells in culture

Journal of Endocrinological Investigation
S KatzburgD Somjen

Abstract

We previously reported a non-enzymatic method for isolation of human bone cells in culture that display osteoblastic features and respond to 1,25 dihydroxy vitamin D (1,25) and to parathyroid hormone (PTH). The present study was undertaken to analyze the response of cultured human bone cells to 17beta-estradiol (E2) and to dihydrotestosterone (DHT) as a function of gender and age. Cultured human bone cells, obtained from biopsies during orthopedic surgery, were divided into four groups defined by gender and age: pre- and post-menopausal healthy non-osteoporotic women that were not under hormone replacement therapy (HRT) and mature (<55-year-old) and older (>60-year-old) men. We found gender specific responses to gonadal steroids using the specific activity of the brain type (BB) isozyme of creatine kinase (CK) as a response marker. Constitutive levels of CK activity did not change with age or gender and the enzyme extracted from cells from the different sexes and ages did not respond to either progesterone (P) or to 1,25. CK from the different cells responded to gonadal steroids in a gender specific manner, i.e. CK from female derived cells responded to E2 only and the enzyme from male derived cells responded to DHT only. In fe...Continue Reading

References

Aug 18, 1978·Calcified Tissue Research·H C van PaassenS A Duursma
Sep 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B P LukertR G Robinson
Jul 15, 1992·Biochemical and Biophysical Research Communications·B A SchevenS A Duursma
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·D SömjenA M Kaye
Jun 1, 1990·Bone and Mineral·J C Gallagher
Nov 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·A M KayeD Sömjen
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D SömjenA M Kaye
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D S ColvardT C Spelsberg
Jan 1, 1986·Advances in Experimental Medicine and Biology·A M KayeD Sömjen
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M ErnstE R Froesch
Sep 15, 1993·Biochemical and Biophysical Research Communications·L L WeiL M Demers
Oct 20, 1994·The New England Journal of Medicine·E P SmithK S Korach
Aug 1, 1993·Calcified Tissue International·H Ben-HurA Ornoy
Feb 1, 1993·Journal of Cellular Physiology·E NasatzkyA Ornoy
Oct 10, 1997·Endocrinology·Y OnoeT Suda
Jul 25, 1998·The Biochemical Journal·M A AnkromN S Fedarko

❮ Previous
Next ❯

Citations

Mar 14, 2013·Journal of Endocrinological Investigation·D SomjenG H Posner
Jun 16, 2010·Biogerontology·Amiela Globerson, Abraham Z Reznick
Oct 19, 2010·The Journal of Steroid Biochemistry and Molecular Biology·D SomjenI Yoles
Sep 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Dalia SomjenSnait Tamir
Apr 20, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Dalia SomjenAlvin M Kaye
Oct 26, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Dalia SomjenIsrael Yoles
Sep 3, 2003·The Journal of Biological Chemistry·Thomas L McCarthyMichael Centrella
May 18, 2006·Proteomics·Adriano SpreaficoAnnalisa Santucci
Jan 18, 2003·Journal of Cellular Biochemistry·Ilka NemerePeter F Blackmore

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.