Age-associated expression of HCN channel isoforms in rat sinoatrial node

Experimental Biology and Medicine
Xin HuangAiqun Ma

Abstract

The expression of hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channel isoforms varies among species, cardiac tissues, developmental stages, and disease generation. However, alterations in the HCN channels during aging remain unclear. We investigated the protein expressions of HCN channel isoforms, HCN1-HCN4, in the sinoatrial nodes (SANs) from young (1-month-old), adult (4-month-old), and aged (30-month-old) rats. We found that HCN2 and HCN4 proteins were present in rat SAN using immunohistochemistry; therefore, we quantitatively analyzed their expression by Western blot. Aim to correlate protein expression and pacemaking function, specific blockade of HCN channels with 3 µmol/L ivabradine prolonged the cycle length in the intact rat heart. During the senescent process, the HCN2 and HCN4 protein levels declined, which was accompanied with a decreased effect of ivabradine on rat SAN automaticity. These results indicated the age-associated expression and relative function of HCN channel isoforms.

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Citations

Feb 9, 2017·Canadian Journal of Physiology and Pharmacology·Xin HuangNing Guo
Nov 23, 2019·Annual Review of Physiology·Colin H PetersCatherine Proenza
Aug 21, 2018·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Iman M Al-NaggarPhillip P Smith
Dec 26, 2018·BioMed Research International·Hong-Bin ZhongLiang-di Xie
May 20, 2020·Pflügers Archiv : European journal of physiology·Ilaria RivoltaJacopo C DiFrancesco
Jul 23, 2021·GeroScience·Sabrina ChoiClaudia M Moreno

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BETA
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SPSS
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