PMID: 6970223Mar 1, 1981Paper

Age-associated increase in expression of the T cell surface markers Thy-1, Lyt-1, and Lyt-2 in congenitally athymic (nu/nu) mice: analysis by flow microfluorometry

The Journal of Immunology : Official Journal of the American Association of Immunologists
H R MacDonaldC Bron

Abstract

The expression of T cell-associated surface markers by lymphoid cells from congenitally athymic (nude) mice has been quantitatively investigated using flow microfluorometry. Spleen and lymph nodes from old (greater than 6 mo) nude mice on either a C57BL/6 or BALB/c genetic background were found to contain significant numbers (5 to 13% in spleen and 15 to 24% in lymph nodes) of cells expressing Thy-1 antigen. The proportion of Thy-1 positive cells in nude spleen was dramatically increased (to 22 to 67%) after passage of the cells over nylon wool columns. In contrast to older animals, young (1- to 2-mo-old) nude mice had undetectable levels (less than 1%) of Thy-1 bearing cells in spleen and reduced levels (6%) in lymph nodes. After passage of their spleen cells over nylon wool, some Thy-1 positive cells (10%) were detectable in 2-mo-old nude mice but none were detectable at 1 mo. In addition to Thy-1, we were able to detect the T cell alloantigens Lyt-1 and Lyt-2 on nylon wool-passed spleen cells from older C57BL/6 or BALB/c nu/nu mice. In general, the proportion of Lyt-1 bearing cells in nude lymphoid populations was similar to the proportion of Thy-1 positive cells. A smaller fraction of nude cells (corresponding to 35 to 59% ...Continue Reading

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