Age-associated potency decline in bovine oocytes is delayed by blocking extracellular Ca(2+) influx

Theriogenology
Shuan ZhaoShen-Ming Zeng

Abstract

Oocyte aging due to delayed fertilization is associated with declining quality and developmental potential. Intracellular calcium (Ca(2+)) concentration ([Ca(2+)]i) regulates oocyte growth, maturation, and fertilization and has also been implicated in aging. Using bovine oocytes, we tested the hypothesis that oocyte aging could be delayed by reducing [Ca(2+)]ivia blocking the influx of extracellular Ca(2+) or chelating ooplasmic free Ca(2+). After IVM, cumulus-oocyte complexes or denuded oocytes were cultured in medium supplemented with 1-octanol, phorbol 12-myristate 13-acetate, or 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis-acetoxymethyl ester (BAPTA-AM) to manipulate [Ca(2+)]i. Addition of 1-mM 1-octanol increased blastocyst development rates in the cumulus-oocyte complexes aged for 6 hours by IVF and for 6, 12, and 24 hours by parthenoactivation, and this effect was independent of the presence of cumulus cells. The intracellular levels of ATP, Glutathione, and Glutathione disulfide were not affected by 1-octanol, but [Ca(2+)]i was significantly decreased. When oocytes were cultured in Ca(2+)-free medium for 12 hours, the blastocyst development rate was greater and the beneficial effects of 1-octanol on...Continue Reading

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Citations

May 14, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Tayita SuttirojpattanaMasaya Geshi
Jul 10, 2018·Reproduction in Domestic Animals = Zuchthygiene·Shuaishuai WuShenming Zeng
Jul 17, 2020·Open Biology·Paula SteinCarmen J Williams
Nov 13, 2021·Frontiers in Cell and Developmental Biology·Lin MengYunpeng Hou

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