PMID: 3758224Aug 1, 1986Paper

Age-dependent changes in nuclear DNA content and cell size of presumably normal human corneal endothelium

Experimental Eye Research
H IkebeS Fujita

Abstract

In order to investigate a relationship between cellular DNA-content and enlargement in cell size of human corneal endothelium in healthy population, both nuclear DNA-cytofluorometry and cell morphometry were performed on individual cells of presumably normal corneas taken from six autopsy cases. The cornea was treated in 0.2 M phosphate buffer containing 60 mM Na2EDTA and fixed with absolute methanol. The endothelial-cell layer was separated from Descemet's membrane by placing the tissue in distilled water. The specimen was marked with rhodamine-labelled wheatgerm agglutinin (WGA)-lectin for identifying cell boundaries and subsequently stained with DAPI for measurement of DNA contents. The area of each cell was measured by means of a color image analyser and compared with its cytofluorometric nuclear DNA content. The enlarged endothelial cells which appeared with age contained diploid, polyploid or multiple nuclei. The polyploid and multinucleated cells had larger mean and maximum cell sizes and more irregular and polymorphous shapes than diploid cells. There were neither small polyploid nor small multinucleated cells. It was suggested that the loss of human corneal endothelium was compensated in vivo by cell size enlargement w...Continue Reading

References

Nov 1, 1978·Archives of Ophthalmology·A LauleC Hanna
Jun 1, 1976·Experimental Eye Research·R A LaingH M Leibowitz
Mar 1, 1976·American Journal of Ophthalmology·W M Bourne, H E Kaufman
Feb 1, 1968·American Journal of Ophthalmology·G Smolin
Jun 1, 1982·Ophthalmology·W F Treffers
Oct 1, 1984·Experimental Eye Research·H IkebeS Fujita
Jan 1, 1983·Histochemistry·S Hamada, S Fujita
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Fujita
Jun 7, 1980·Journal of Theoretical Biology·H Honda, G Eguchi

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Citations

Jul 11, 2003·Progress in Retinal and Eye Research·Nancy C Joyce
Nov 19, 2013·Trends in Cell Biology·Yoichiro Tamori, Wu-Min Deng
Aug 10, 2013·Aging Cell·Danny S RohJames L Funderburgh
Aug 1, 1988·Experimental Eye Research·H IkebeS Fujita
Jun 9, 2016·Advances in Wound Care·Vicki P Losick
Oct 15, 2020·ELife·Nora G PetersonDonald T Fox

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