Oct 30, 2018

Age-dependent changes in transcription factor FOXO targeting in Drosophila melanogaster

BioRxiv : the Preprint Server for Biology
Allison BirnbaumHua Bai

Abstract

FOXO transcription factors have long been associated with longevity and tissue homeostasis. Although direct targets of FOXO have been reported in various long-lived mutants or stress conditions (nutrient deprivation), no studies have investigated how normal aging impacts the transcriptional activity of FOXO. Here, we conducted chromatin immunoprecipitation sequencing in both young and old wild-type fruit flies, Drosophila melanogaster, to evaluate the dynamics of FOXO gene targeting during aging. Drosophila FOXO binds to 2617 genes at young ages, whereas the number of FOXO-bound genes dramatically decreases to 224 at old ages. Consistently, many genes bound by FOXO in young flies are transcriptionally altered with age, including genes in Hippo and MAPK signaling pathways. Interestingly, we also find that many FOXO-bound genes in wild-type flies are unique from those in insulin mutants, suggesting FOXO is regulated by multiple upstream inputs. Together, these results provide new insights into dynamic FOXO targeting under normal aging and highlight the diverse regulatory mechanisms for FOXO transcriptional activity that are currently less understood.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Study
Biochemical Pathway
Genes
Insulin/Insulin Receptor Signaling Pathway
Transcription, Genetic
Mapk Signaling
Mutant Proteins
Drosophila
Nutrients
Chromatin Immunoprecipitation

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Cell Aging (Preprints)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.