PMID: 3753091Jul 1, 1986Paper

Age-dependent decrease of the lateral diffusion constant of proteins in the plasma membrane of hepatocytes as revealed by fluorescence recovery after photobleaching in tissue smears

Archives of Gerontology and Geriatrics
I Zs-NagyK Imahori

Abstract

When fresh liver is smeared on slides and incubated in Krebs-Henseleit Ringer solution containing 1 mM H2O2 at 37 degrees C, a yellowish-green autofluorescence develops in the hepatocyte plasma membrane. Indirect evidence shows that this peroxide-induced autofluorescence (PIAF) is due most probably to chemical reactions between proteins and the malondialdehyde produced by the membrane lipid peroxidation. Although the chemical nature of the PIAF has not been clarified yet, it is suitable under certain conditions for the measurement of the average lateral diffusion constant of the membrane proteins by means of the fluorescence recovery after photobleaching (FRAP) technique without the addition of any external fluorescent label. Analysis of four age groups for both sexes of Fischer 344 rats (3-5 rats per group, total 32) from 2 to 31 months of age revealed a significant negative linear correlation of the lateral diffusion constant of proteins with age in both sexes, with the slope of the females being somewhat smaller. Young males showed a diffusion constant about 2.8 X 10(-10) decreasing to 1.7 X 10(-10) cm2 X s-1 by 31 months of age at 37 degrees C, whereas the respective values in females were 2.7 X 10(-10) and 1.9 X 10(-10). T...Continue Reading

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Citations

Apr 22, 1988·Biochimica Et Biophysica Acta·M NokuboM Ohta
Oct 19, 1992·Journal of Immunological Methods·E N Kaufman, R K Jain
Jan 1, 1988·Mechanisms of Ageing and Development·P BaldiniP Luly
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Mar 1, 1991·Archives of Gerontology and Geriatrics·K Kitani
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Mar 1, 1991·Archives of Gerontology and Geriatrics·I Zs -Nagy
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Sep 1, 1994·Archives of Gerontology and Geriatrics·K Kitani
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Feb 7, 2004·Archives of Gerontology and Geriatrics·Imre Zs-Nagy
Dec 12, 2001·Experimental Gerontology·S DamjanovichR Gáspár
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Jun 30, 1994·Annals of the New York Academy of Sciences·I Zs-Nagy
Jan 1, 1988·Annals of the New York Academy of Sciences·I Zs-NagyI Semsei
Dec 18, 2002·Annals of the New York Academy of Sciences·Tamas FulopGilles Dupuis

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