Age-dependent increases in protein kinase C-alpha beta immunoreactivity and activity in the human brain: possible in vivo modulatory effects on guanine nucleotide regulatory G(i) proteins

Brain Research
X BusquetsJ A Garcia-Sevilla

Abstract

In the postmortem human brain (20 specimens of frontal cortex, Brodmann area 9) the abundance of immunoreactive protein kinase C (PKC-alpha beta) and the activity of PKC (calcium, phosphatidylserine, and phorbol ester-dependent enzymes) were determined to study the effect of aging (range 1 month to 89 years) on this regulatory enzyme. Also, the abundance of immunoreactive G protein subunits (G alpha i1/2, G alpha i3, G alpha o, G alpha s and G beta) were assessed in parallel to investigate possible relationships with PKC-alpha beta. The abundance of PKC-alpha beta was positively correlated with aging (r = 0.62, n = 20, P < 0.005). Moreover, PKC activity also showed a significant positive correlation with the age of the subject at death (r = 0.55, n = 14, P < 0.05). Because of the known in vitro modulatory role of PKC-alpha beta on G(i) proteins, the existence of an in vivo effect of brain PKC-alpha beta on various G proteins was assessed through correlation analyses. In the brain of the same subjects, there were significant negative correlations between the immunoreactivity of PKC-alpha beta and the immunoreactivities of G alpha i1/2 (r = -0.78, n = 13, P < 0.005) and G alpha i3 (r = -0.58, n = 15, P < 0.005). In the same brain...Continue Reading

References

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Citations

Jun 29, 2000·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·M A CoullR W Horton
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·A NogueraA G Agustí
Oct 6, 2017·JCI Insight·Youichi ShinozakiSchuichi Koizumi
Aug 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·D M Boesch, J L Garvin
Apr 17, 2021·Trends in Endocrinology and Metabolism : TEM·Devanshi Mishra, Chinmoy Sankar Dey

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