Age-dependent susceptibility of the retinal ganglion cell layer to cell death

Investigative Ophthalmology & Visual Science
Declan P McKernanThomas G Cotter

Abstract

The purpose of this study was to determine the susceptibility of the retinal ganglion cell layer (GCL) to apoptosis after optic nerve transection and excitotoxic stimulus and to investigate the regulation of apoptosis in the GCL during development. The authors also sought to determine the role played by caspases in cell death and their expression during development. TdT-mediated dUTP nick end labeling (TUNEL) was used to identify cells undergoing apoptosis during mouse retinal development from postnatal day (P)3 to P5 and in retinal explant sections under various conditions. The expression of active caspases was determined by immunohistochemistry (IHC) using an antibody that detects the cleaved large subunit. IHC was also used to detect the expression levels of procaspase-3, procaspase-9, and Apaf-1 in P6 and P60 whole eye sections. Retinal ganglion cells at ages P6 and P60 were purified by immunopanning, total RNA was extracted, and mRNA levels of the above proteins were determined by semiquantitative PCR. After optic nerve transection, a significant number of TUNEL-positive cells were seen 24 hours after lesion in P6 retinas. This death was caspase dependent, as shown by IHC and caspase inhibition with zVAD-fmk. In contrast, ...Continue Reading

Citations

Feb 9, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Massimo Dal MontePaola Bagnoli
Jun 29, 2013·Cell Death & Disease·A J KoleM Deshmukh
Jan 28, 2014·Biochemistry. Biokhimii︠a︡·O S GancharovaP P Philippov
Sep 14, 2010·Experimental Eye Research·Lilian KisiswaMichael A Wride
Apr 20, 2007·Journal of Neurochemistry·Declan P McKernan, T G Cotter
Apr 15, 2011·Clinical & Experimental Ophthalmology·Marc B GuerinThomas G Cotter
Oct 3, 2006·Experimental Eye Research·Carolyn O'DriscollThomas G Cotter
Apr 14, 2016·Experimental Eye Research·Sonia T HaneaPatrice D Smith
Dec 26, 2013·Experimental Eye Research·Matthew R SteinhartHarry A Quigley
Mar 25, 2008·General and Comparative Endocrinology·Esmond J SandersSteve Harvey
Sep 10, 2016·PloS One·Patrik Maximilian BauerUlrica Englund-Johansson
Aug 31, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C SternbergR Linden
Jun 24, 2019·The FEBS Journal·Emilie HollvilleMohanish Deshmukh
Jun 27, 2019·Alternatives to Laboratory Animals : ATLA·Sven SchnichelsStephanie C Joachim
Oct 27, 2021·Cellular and Molecular Neurobiology·Wang ShengJian-Su Chen

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