PMID: 15374391May 1, 1992Paper

Age-dependent variations of human PMNL elastase activity as a function of pH, ionic strength and calcium concentration

Archives of Gerontology and Geriatrics
Z VargaT Fülöp

Abstract

Human leukocyte elastase (HLE) has been implicated in the pathomechanism of various diseases, such as emphysema and atherosclerosis. The incidence of these diseases is increasing with aging. Therefore, it can be supposed that the HLE activity is changing with aging according to the well known age-related physiological alterations. Thus, the effects of pH, NaCl and calcium concentrations on HLE activity, separated from polymorphonculear leucocytes (PMNLs) of healthy, young (<35 years) and elderly (>75 years) subjects, were studied by measuring the activity with synthetic substrate and with bovine and human atherosclerotic and non-atherosclerotic aortic elastin. From our results, it may be concluded, that the elastolytic activity of HLE separated from PMNLs of elderly subjects is more sensitive to ionic strength, to pH and to the calcium concentration of the medium, than the HLE activity of young subjects. The elastolytic activity of HLE, of both young and old subjects, increases dramatically on atherosclerotic aortic elastin in the presence of calcium. These findings might explain, at least partly, the increased incidence of atherosclerosis with aging.

References

Jan 1, 1978·Experimental Gerontology·W HornebeckL Robert
Feb 3, 1975·European Journal of Biochemistry·W Hornebeck, S M Partridge
Sep 9, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·J M SaulnierJ M Wallach
Mar 1, 1989·Archives of Gerontology and Geriatrics·T FülöpA Leövey
Feb 20, 1986·The New England Journal of Medicine·R Ross
Dec 1, 1986·Atherosclerosis·J Nilsson
Mar 1, 1987·Age and Ageing·R W Stout
Oct 1, 1986·The Journal of Experimental Medicine·R J HaririM E Weksler
Jan 1, 1985·Mechanisms of Ageing and Development·T FülöpA Leövey
Apr 16, 1985·Biochemical and Biophysical Research Communications·W C GroutasJ P Crowley
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D W Urry
Jan 1, 1983·Connective Tissue Research·V GuantieriD D Gordini
Aug 15, 1984·Archives of Biochemistry and Biophysics·G OshimaM Yamada
Apr 1, 1980·Biochemical Medicine·C BoudierJ G Bieth
Mar 5, 1981·The New England Journal of Medicine·L B SandbergJ G Leslie
Nov 21, 2012·Current Opinion in Clinical Nutrition and Metabolic Care·Tommy Cederholm, John Morley

❮ Previous
Next ❯

Citations

May 19, 2017·Frontiers in Cellular and Infection Microbiology·Elsa N Bou GhanemAlexander Panda
Aug 14, 2021·Beilstein Journal of Organic Chemistry·Renato L CarvalhoEufrânio N da Silva Júnior

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.