PMID: 15377893Sep 21, 2004Paper

Age disproportionately increases sepsis-induced apoptosis in the spleen and gut epithelium

Shock
Isaiah R TurnbullCraig M Coopersmith

Abstract

Both aging and sepsis independently increase splenic and gut epithelial apoptosis. Sepsis-induced apoptosis in either cell type is also associated with increased mortality in young mice. We sought to determine whether age alters sepsis-induced splenic and gut epithelial cell death. Young (2 months) and aged (22 months) male ND4 mice were subjected to either single-puncture cecal ligation and puncture (CLP) with a 23-gauge needle or sham laparotomy. Apoptosis was assessed 24 hours later in the spleen and gut epithelium by active caspase 3 and hematoxylin and eosin staining. Aged septic mice had increased splenic apoptosis compared with either young septic animals or aged sham animals (15 vs. 7 vs. 5 apoptotic cells/high-powered field, P < 0.05). Similarly, aged septic animals had an elevation in gut epithelial cell death compared with either young septic or aged sham mice (33 vs. 16 vs. 6 apoptotic cells/100 contiguous crypts, P < 0.05). Elevated intestinal cell death was not associated with changes in either gut proliferation or cell division. To verify that the increase in splenic apoptosis seen in septic aged animals was not strain specific, double-puncture CLP with a 25-gauge needle or sham laparotomy was performed on young ...Continue Reading

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Citations

Jun 1, 2005·The Journal of Surgical Research·Pardis JavadiCraig M Coopersmith
Aug 3, 2013·The Journal of Infectious Diseases·Yijie ZhengYang Jin
Dec 19, 2006·Shock·Patrice PétillotRémi Neviere
Feb 25, 2009·Critical Care Medicine·Isaiah R TurnbullCraig M Coopersmith
Dec 17, 2009·Critical Care Medicine·Amy C FoxCraig M Coopersmith
Aug 6, 2008·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Paul A ChecchiaJ Perren Cobb
Jul 19, 2008·Shock·Vanessa NomelliniElizabeth J Kovacs
Jun 11, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dinesh VyasCraig M Coopersmith
Sep 24, 2013·Trends in Molecular Medicine·Rohit Mittal, Craig M Coopersmith
Mar 21, 2007·World Journal of Surgery·Evangelos MessarisManousos M Konstadoulakis
Dec 1, 2015·Experimental Gerontology·Alice Elizabeth KaneSarah Nicole Hilmer
Nov 28, 2009·Best Practice & Research. Clinical Gastroenterology·Alan B R Thomson
Nov 7, 2009·Critical Care Clinics·Sergio L Zanotti-Cavazzoni, Roy D Goldfarb
Mar 31, 2015·Ageing Research Reviews·Jung-Woo KangSun-Mee Lee
Jan 17, 2017·Current Opinion in Critical Care·Mei MengCraig M Coopersmith
Sep 17, 2016·Experimental Biology and Medicine·Javier Cabrera-PerezThomas S Griffith
Feb 8, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin W McConnellCraig M Coopersmith
Apr 27, 2017·ILAR Journal·Julie A StortzPhilip A Efron
Jul 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Javier Cabrera-PerezThomas S Griffith
Feb 4, 2021·Aging and Disease·Wanxue HeLixin Xie

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis