Age Increases Monocyte Adhesion on Collagen

Scientific Reports
Samira KhalajiKay-E Gottschalk

Abstract

Adhesion of monocytes to micro-injuries on arterial walls is an important early step in the occurrence and development of degenerative atherosclerotic lesions. At these injuries, collagen is exposed to the blood stream. We are interested whether age influences monocyte adhesion to collagen under flow, and hence influences the susceptibility to arteriosclerotic lesions. Therefore, we studied adhesion and rolling of human peripheral blood monocytes from old and young individuals on collagen type I coated surface under shear flow. We find that firm adhesion of monocytes to collagen type I is elevated in old individuals. Pre-stimulation by lipopolysaccharide increases the firm adhesion of monocytes homogeneously in older individuals, but heterogeneously in young individuals. Blocking integrin αx showed that adhesion of monocytes to collagen type I is specific to the main collagen binding integrin αxβ2. Surprisingly, we find no significant age-dependent difference in gene expression of integrin αx or integrin β2. However, if all integrins are activated from the outside, no differences exist between the age groups. Altered integrin activation therefore causes the increased adhesion. Our results show that the basal increase in integri...Continue Reading

References

Jun 1, 1993·Current Opinion in Immunology·N Hogg, R C Landis
Mar 23, 2000·The Journal of Clinical Investigation·A R de FougerollesV E Koteliansky
Mar 21, 2001·Cellular Signalling·M Guha, N Mackman
Mar 30, 2001·The Journal of Biological Chemistry·N AshidaT Kita
Apr 6, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jesse RomanSusanne Roser-Page
Jan 16, 2009·Vascular Medicine·Eser AdiguzelMichelle P Bendeck
Jun 23, 2009·Trends in Immunology·Alexander PandaAlbert C Shaw
Oct 11, 2011·Nature Reviews. Immunology·Chao Shi, Eric G Pamer
Feb 14, 2012·Immunobiology·Alexei GratchevJulia Kzhyshkowska
Jan 25, 2013·Veterinary Pathology·W A Muller
Apr 2, 2013·The International Journal of Biochemistry & Cell Biology·Matti LahtiJarmo Käpylä
Oct 17, 2013·The Journal of Biological Chemistry·Liisa M UotilaCarl G Gahmberg
May 21, 2015·Cardiovascular Research·Teresa Gerhardt, Klaus Ley
Feb 26, 2016·Acta Neuropathologica·Lisa ZondlerJochen H Weishaupt

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Citations

Apr 4, 2021·Cells·Jordan HollAndrzej Eljaszewicz
Jul 15, 2021·Immunity·Nikola Makdissi, Elvira Mass

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Methods Mentioned

BETA
FCS

Software Mentioned

ImageJ
GraphPad PRISM

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