Age of donor alters the effect of cyclic hydrostatic pressure on production by human macrophages and osteoblasts of sRANKL, OPG and RANK

BMC Musculoskeletal Disorders
C E EvansJ G Andrew

Abstract

Cyclic hydrostatic pressure within bone has been proposed both as a stimulus of aseptic implant loosening and associated bone resorption and of bone formation. We showed previously that cyclical hydrostatic pressure influenced macrophage synthesis of several factors linked to osteoclastogenesis. The osteoprotegerin/soluble receptor activator of NF-kappa beta ligand /receptor activator of NF-kappa beta (OPG/ RANKL/ RANK) triumvirate has been implicated in control of bone resorption under various circumstances. We studied whether cyclical pressure might affect bone turnover via effects on OPG/ sRANKL/ RANK. In this study, cultures of human osteoblasts or macrophages (supplemented with osteoclastogenic factors) or co-cultures of macrophages and osteoblasts (from the same donor), were subjected to cyclic hydrostatic pressure. Secretion of OPG and sRANKL was assayed in the culture media and the cells were stained for RANK and osteoclast markers. Data were analysed by nonparametric statistics. In co-cultures of macrophages and osteoblasts, pressure modulated secretion of sRANKL or OPG in a variable manner. Examination of the OPG:sRANKL ratio in co cultures without pressurisation showed that the ratio was greater in donors <70 years a...Continue Reading

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Citations

Mar 1, 2008·Apoptosis : an International Journal on Programmed Cell Death·Letizia PenolazziRoberta Piva
May 13, 2006·The International Journal of Biochemistry & Cell Biology·C E EvansJ G Andrew
Nov 19, 2011·Journal of Clinical Periodontology·Valdir Gouveia GarciaLetícia Helena Theodoro
Nov 10, 2009·Journal of Dental Research·Y SuzukiO Suzuki
Jan 10, 2018·Journal of Biomedical Materials Research. Part a·Ryosuke IwamaOsamu Suzuki
Aug 1, 2009·Tissue Engineering. Part a·Aritsune MatsuiOsamu Suzuki

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