PMID: 2106292Feb 15, 1990Paper

Age-related change in the degradation rate of ovalbumin microinjected into mouse liver parenchymal cells

Archives of Biochemistry and Biophysics
A Ishigami, S Goto

Abstract

Change in the degradation rate of ovalbumin (OVA) microinjected into liver parenchymal cells isolated from mice of various ages was studied. OVA was injected by osmotic lysis of pinosomes and the amount of OVA was determined by immunoblotting using purified antibody to OVA. Cellular activity as judged by rates of protein synthesis and degradation of pulse-labeled proteins was not affected by the injection. To localize injected OVA in the cells, cell extracts were fractionated by differential centrifugation and the amount of OVA and the activity of beta-D-galactosidase as a lysosomal marker enzyme of each fraction were determined. The ovalbumin was mostly found in the soluble fraction, while the beta-D-galactosidase activity was found in the particulate fraction, indicating that the ovalbumin was spread in the cytosol but was not present in the pinosomes or lysosomes. The average half-lives of OVA were 106, 113, and 164 h in the cells from young (3.5-6.5 months), middle-aged (13.5-20.0 months), and old (24.5-30.5 months) mice, respectively. Thus, the half-life of ovalbumin in the cells of senescent mice was about 50% longer than that in the cells of young or middle-aged mice. These results are in good agreement with those of our...Continue Reading

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Citations

Oct 13, 1995·Mechanisms of Ageing and Development·F Sierra
May 1, 1993·Archives of Gerontology and Geriatrics·S MorimotoS Goto
Sep 1, 1994·Archives of Gerontology and Geriatrics·S Goto
Jun 11, 1998·Mechanisms of Ageing and Development·M M ScrofanoA Taylor
Jul 15, 1998·Mechanisms of Ageing and Development·T Hayashi, S Goto
Sep 9, 2000·Experimental Gerontology·A J Lambert, B J Merry
Apr 27, 2007·Annals of the New York Academy of Sciences·Sataro GotoRamesh Sharma
Nov 16, 2004·Mechanisms of Ageing and Development·Vincent J CristofaloMaria Tresini
Apr 6, 2005·Biochemical and Biophysical Research Communications·Ming Chen, Hugo L Fernandez
Nov 9, 2002·Microscopy Research and Technique·Ryoya Takahashi, Sataro Goto
Nov 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W Ward, A Richardson
Apr 27, 2002·Annals of the New York Academy of Sciences·Sataro GotoHideko Nakamoto

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