Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation

International Journal of Molecular Sciences
Haojie ZhangYong-Liang Zhang

Abstract

The growth hormone/insulin-like growth factor 1 (GH/IGF-1) axis has recently been recognized as an important factor related to the longevity of many organisms. MicroRNAs (miRNAs or miRs) could also participate in diverse biological processes. However, the role of miRNAs in the decline of pituitary GH during the growth process remains unclear. To better characterize the effects of miRNAs on the pituitary, we used a miRNA microarray to investigate the miRNA profile in the rat pituitary from postnatal development throughout the growth process. Then, in vitro experiments were conducted to analyze the miRNAs' potential roles related to GH regulation. Taken together, the microarray results indicated that there were 22 miRNAs differentially expressed during pituitary development. The bioinformatics analysis suggested that the most differentially expressed miRNAs may participate in multiple pathways associated with the pituitary function. Furthermore, the in vitro findings demonstrated that miR-141-3p was involved in GH regulation.

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Citations

Nov 29, 2020·Endocrinology·Mary P LaPierreMarkus Stoffel
Mar 7, 2021·Animals : an Open Access Journal From MDPI·ZeWen YuBao Yuan
Jan 9, 2020·Hormone Research in Pædiatrics·Francesca CirilloMaria Elisabeth Street

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Methods Mentioned

BETA
chip
PCR
electrophoresis
Protein Assay

Software Mentioned

ingenuity pathway analysis ( IPA )
SPSS Statistics
Excel
miRanda
IPA
Cluster
TargetScan
Image JSoftware
Cytoscape

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