Age-related changes in Na, K-ATPase expression, subunit isoform selection and assembly in the stria vascularis lateral wall of mouse cochlea

Hearing Research
Bo DingRobert D Frisina

Abstract

Due to the critical role of cochlear ion channels for hearing, the focus of the present study was to examine age-related changes of Na, K-ATPase (NKA) subunits in the lateral wall of mouse cochlea. We combined qRT-PCR, western blot and immunocytochemistry methodologies in order to determine gene and protein expression levels in the lateral wall of young and aged CBA/CaJ mice. Of the seven NKA subunits, only the mRNA expressions of α1, β1 and β2 subunit isoforms were detected in the lateral wall of CBA/CaJ mice. Aging was accompanied by dys-regulation of gene and protein expression of all three subunits detected. Hematoxylin and eosin (H&E) staining revealed atrophy of the cochlear stria vascularis (SV). The SV atrophy rate (20%) was much less than the ∼80% decline in expression of all three NKA isoforms, indicating lateral wall atrophy and NKA dys-regulation are independent factors and that there is a combination of changes involving the morphology of SV and NKA expression in the aging cochlea which may concomitantly affect cochlear function. Immunoprecipitation assays showed that the α1-β1 heterodimer is the selective preferential heterodimer over the α1-β2 heterodimer in cochlea lateral wall. Interestingly, in vitro pathway e...Continue Reading

Citations

Feb 7, 2020·Journal of Neuroscience Research·Tanika T WilliamsonRobert D Frisina
May 9, 2019·Journal of Neuroscience Research·Elizabeth M Keithley
Dec 19, 2020·Pflügers Archiv : European journal of physiology·Barbara Peixoto PinheiroYoussef Adel
May 8, 2021·Frontiers in Molecular Neuroscience·Luis Colón-CruzMartine Behra
Jul 3, 2021·International Journal of Molecular Sciences·Parveen BazardBo Ding

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