Nov 1, 1981

Age-related changes in the glycosaminoglycans and collagen of cultured pig endothelial cells

M Bihari-VargaH Jellinek


Pig aortic endothelial cells were grown from aortic intimal explants. Tissue culture samples were obtained at regular intervals between the 2nd and 21st day (time-course experiments) and between the 2nd and 15th passage (influence of subcultivation). Glycosaminoglycans (GAG-s) were isolated separately from the cells and from the medium. The various GAG-s were identified by electrophoresis on cellulose acetate strips, by differential degradations with HNO2 and by digestion with selective enzymes, and their concentration was measured. Protein and collagen content was also determined. Parallel tissue culture monolayers were investigated by microscopical methods. Total GAG and collagen concentrations showed maximum curves when plotted as a function of the time of incubation, with peak values on the 9th day, both in the cells and in the medium. From among the individual GAG-s the amount of hyaluronic acid and chondroitin-sulfate increased gradually, while heparan-sulfate content changed in the opposite direction. Chondroitin was detectable only in the culture medium; its concentration decreased steeply until the tenth day. Alterations in the dermatansulfate content showed inverse tendency. The production and excretion of collagen an...Continue Reading

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Microbial Subculture
Enzymes, antithrombotic
Hyaluronic Acid Test
Hyaluronic Acid

About this Paper

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.