Age-related changes in the properties of the endothelin receptor system at protein and mRNA levels in the rat vas deferens

Journal of Receptor and Signal Transduction Research
M YonoR M Weiss

Abstract

As age-related changes occur in the properties of the endothelin (ET) receptor system in several mammalian tissues, and as there are significant amounts of functional ET receptors in the vas deferens, we investigated the age-related changes in the ET receptor system at the protein and mRNA levels in the rat vas deferens. The ET system was investigated in the vasa deferentia of 3 weeks, 3 months and 22 months old rats. ET receptors were characterized and quantified at the protein level by radioligand receptor binding, and gene transcript levels of ET-1, ET-3, ET converting enzyme-1 (ECE-1), and ET(A) and ET(B) receptor subtypes were quantified by real-time reverse transcription polymerase chain reaction (RT-PCR). The results of radioligand receptor binding assays demonstrate that there is a higher density of total ET receptors in the vas deferens of 3 weeks old rats than in 3 months and 22 months old rats, and that the predominant ET receptor is of the ET(A) subtype in all three ages. Real-time RT-PCR data show that the predominant mRNA expression of ETs and their receptors in all age groups studied are ET-1 and the ET(A) receptor subtype, respectively. Furthermore, ET-1, ET-3, ECE-1, and ET(A) and ET(B) receptor subtype mRNAs a...Continue Reading

References

Dec 22, 1999·Neurourology and Urodynamics·M SaitoR M Weiss
Jul 25, 2000·BJU International·M E SullivanR J Morgan
Jun 23, 2001·Drugs & Aging·M P Love, J J McMurray
Nov 1, 2001·Journal of Pharmacological and Toxicological Methods·T D Westfall, D P Westfall
Apr 5, 2002·Trends in Pharmacological Sciences·Anthony P Davenport, Janet J Maguire
Jul 9, 2002·Investigational New Drugs·E Scott KopetzMichael A Carducci
Apr 18, 2003·Naunyn-Schmiedeberg's Archives of Pharmacology·Parviz AfiatpourRobert M Weiss
Oct 21, 2003·Journal of Pharmacological and Toxicological Methods·Makoto YonoJamshid Latifpour

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