Age-related changes of mitogen responsiveness in different lymphoid organs from outbred NMRI mice

Immunobiology
F JoncourtA L de Weck

Abstract

Lymphocytes from spleen, thymus and lymph nodes from individual young adult (3--4 months) and aged (26--30 months) NMRI mice were stimulated with the mitogens Con A, PHA and LPS. 24 hours later, the number of cell with increased RNA-content (G1 cells) was determined by cytofluorometry. In parallel the 3H-thymidine incorporation after 48 hours was measured for the same cell samples. Aged animals in average produced less G1 cells and incorporated less 3H-thymidine as compared to young adults. By calculating the 3H-thymidine incorporation per G1 cell, the proliferative capacity of mitogen-induced G1 cells can be estimated. These ratios are lower in aged mice as compared to young adult, suggesting that in these animals not only less cells can be activated as measured by cytofluorometry, but also from these activated cells again fewer continue the cell cycle by initiating DNA-synthesis. In response to Con A and PHA, aged mice in average produce less G1 cells in all of the three lymphoid organs tested. In response to LPS, however, the young adult produced only few G1 cells in lymph nodes and practically none in thymus, whereas in aged animals a considerable number of G1 cells was found in both organs. Corresponding results were found...Continue Reading

References

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Citations

Jul 14, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Aspinall
Jun 5, 2002·Mechanisms of Ageing and Development·Hui-Chen HsuGary Van Zant
Jul 16, 1999·Mechanisms of Ageing and Development·B Chakravarti, G N Abraham
May 15, 2007·Cellular Immunology·Ana LustigDennis D Taub
Apr 1, 1984·Cellular Immunology·D O SlausonA L de Weck
Aug 1, 1987·Journal of Cellular Physiology·R A MillerE R Simons
Feb 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Sam PalmerT J Newman
Jan 1, 1988·Annals of the New York Academy of Sciences·K W KelleyS Arkins
May 11, 2005·Immunological Reviews·Dennis D Taub, Dan L Longo
Dec 22, 2004·Mechanisms of Ageing and Development·Hui-Chen HsuJohn D Mountz

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