Age-related differences in oxidative protein-damage in young and senescent fibroblasts

Archives of Biochemistry and Biophysics
Tobias JungT Grune

Abstract

Aging is accompanied by an accumulation of oxidized proteins and cross-linked modified protein material. The intracellular formation and accumulation of highly oxidized and cross-linked proteins, the so-called lipofuscin, is a typical sign of senescence. However, little is known whether the lipofuscin accumulation during aging is related to environmental conditions, as oxidative stress, and whether the accumulation of oxidized proteins and lipofuscin is preferentially taking place in the cytosol or the nucleus and finally, what is the role of lysosomes in this process. Therefore, we investigated human skin fibroblasts in an early stage of proliferation ("young cells") and in a late stage ("senescent cells"). Such cells were compared for the amount of protein carbonyls and lipofuscin and their distribution within the cytosol and the nucleus. Furthermore, cells were exposed to single and repeated doses of hydrogen peroxide and paraquat, measuring the same set of parameters. In addition to that the role of the proteasome to degrade oxidized proteins in young and senescent cells was tested. Furthermore, detailed microscopic analysis was performed testing the intracellular distribution of lipofuscin. The results clearly demonstrated...Continue Reading

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Citations

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Feb 26, 2020·Free Radical Biology & Medicine·Tilman Grune
Jul 1, 2018·Free Radical Biology & Medicine·Laura C D Pomatto, Kelvin J A Davies
Apr 26, 2019·Redox Biology·Laura C D PomattoKelvin J A Davies
Aug 2, 2017·Redox Biology·Ioanna KorovilaTobias Jung
May 16, 2021·Journal of Cosmetic Dermatology·Changhui ChoJaesung Hwang
Oct 27, 2009·Free Radical Biology & Medicine·Tobias JungTilman Grune

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