Age-related effects of cocultured rat nucleus pulposus cells and macrophages on nitric oxide production and cytokine imbalance

Spine
Raijiro KatsunoJoji Mochida

Abstract

A study of age-related effects on nitric oxide (NO) and cytokine production in cocultured rat nucleus pulposus (NP) cells and macrophages. To evaluate the effects of age on NO and cytokine production in an in vitro model of cocultured NP cells and macrophages. It is well known that the clinical characteristics of lumbar disc herniation differ with age. The relationship between age-related differences in clinical features and immuno-chemical factors, such as NO and inflammatory cytokines, has not been established. Male Sprague Dawley rats (n = 45), including 15 animals from 3 groups (3-, 12-, and 32-weeks old), were used. NP cells and exudated peritoneal macrophages were cocultured in serum-free media. NO levels were measured at 2-, 24-, 48-, and 72 hours using the Griess method. After 7 days of culture, the production of cytokines, including tissue inhibitor metalloproteinase-1, interferon-gamma (IFN-gamma), and interleukin-10 (IL-10) were evaluated. NO levels of coculture increased with age. In the coculture groups, tissue inhibitor metalloproteinase-1 and IFN-gamma level of 3 weeks old were statistically higher than 12 and 32 weeks old. IL-10 level of 3 weeks old was statistically lower than 12 and 32 weeks old. NO levels of ...Continue Reading

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Citations

Sep 16, 2009·Spine·Matthias SchulzVenkataraman Balakrishnan
Sep 3, 2013·Expert Opinion on Therapeutic Targets·Alberto Di MartinoCesare Faldini
Feb 23, 2011·Medical Hypotheses·Xin-Long MaChao Han
Mar 12, 2011·Journal of the American Geriatrics Society·Pradeep SuriJames Rainville
Apr 18, 2015·Journal of Orthopaedic Surgery and Research·Chao HanXiao-Dan Li
Jul 29, 2015·The American Journal of Pathology·Manuela R VerasteguiRobert H Gilman
Feb 14, 2018·International Journal of Molecular Medicine·Kang LiJie Zhao

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