Age-related effects of ectopic pituitary transplants on the activation of Ames dwarf mouse lymphocytes in vitro

Proceedings of the Society for Experimental Biology and Medicine
A I EsquifinoAndrzej Bartke

Abstract

Prolactin (PRL), one of the anterior pituitary hormones, has been implicated in the development and maintenance of immune system function. The following experiments were conducted to evaluate age-related effects of PRL on immune system activity in a PRL-deficient mouse model, the Ames dwarf. Two- and ten-month-old dwarf and phenotypically normal male mice were used. Six dwarf mice from each age group received a surgically implanted pituitary (under the kidney capsule) from a normal donor female mouse. Six different dwarfs were similarly operated but had no pituitary graft inserted and will be referred to as sham-operated dwarfs. Three weeks following surgery, spleens were removed from the dwarfs and age-matched normal mice and splenocytes isolated. The two age groups will subsequently be referred to as "3-month-old" and "11-month-old", respectively, The splenocytes were used in mitogen-induced (concanavalin A (Con A); phytohemagglutinin (PHA); lipopolysaccharide (LPS)) proliferation assays, and histopaque isolated lymphocytes were used for T cell surface marker determination. Pituitary grafting increased body weights of dwarf mice although the weights were less than control mice independent of age. A similar pattern was observe...Continue Reading

Citations

Feb 27, 2003·Journal of Physiology and Biochemistry·A I EsquifinoA Bartke
Jan 1, 2000·Journal of the American Aging Association·J A MattisonA Bartke
Oct 4, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R Michael McClainJochen Bausch
May 16, 2015·Blood Cells, Molecules & Diseases·Maegan L CapitanoHal E Broxmeyer
Apr 17, 2013·Physiological Reviews·Andrzej BartkeValter Longo
Jul 3, 2002·Experimental Biology and Medicine·Mary A HallJohn M Martinko
Feb 7, 2020·Physiology·Rochelle BuffensteinHolly M Brown-Borg
Nov 5, 1998·Neurosurgery·M MaxwellP M Black

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