Age-related loss of calcitriol stimulation of phosphoinositide hydrolysis in rat skeletal muscle

Molecular and Cellular Endocrinology
M M FacchinettiA R de Boland

Abstract

We have examined the effects in vitro of calcitriol [1,25(OH)2D3], the hormonal form of vitamin D3, on the breakdown of membrane phosphoinositides in skeletal muscle from young (3 months) and aged (24 months) rats. Calcitriol (10(-9) M) induced a rapid and transient release of IP3/inositol phosphates and diacylglycerol (DAG) from muscle slices/membranes prelabeled with [3H]myo-inositol and [3H]arachidonate, respectively. Inositol phosphate release was maximal at 15 s and then declined. The effects of hormone specificity exhibited as the closely related derivatives of vitamin D3, 25OHD3, 1alphaOHD3 and 24,25(OH)2D3 did not alter muscle inositol phosphate levels. The stimulation of DAG was biphasic, the early phase (15 s) being abolished by neomycin (0.5 mM), an inhibitor of phosphoinositide hydrolysis, similar to IP3 formation and consistent with a role of phospholipase C (PLC) in intracellular signal generation. Neomycin had no effect on the second DAG peak (2 min) induced by calcitriol, suggesting that the late phase of DAG formation is independent from the hydrolysis of phosphoinositides. Higher basal inositol phosphate and DAG levels were detected in muscle from aged rats thereby reducing the effects of the hormone on second...Continue Reading

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Citations

Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Verónica González Pardo, Ana Russo de Boland
Oct 8, 1999·Molecular and Biochemical Parasitology·C M OchattG A Cross
Apr 3, 2003·Experimental Gerontology·Nataliya S KavokNataliya A Babenko
Aug 14, 2002·The British Journal of Dermatology·J E Osborne, P E Hutchinson
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·T A HornbergerS Chien
Jul 21, 2006·Biogerontology·Verónica Gonzalez PardoAna Russo de Boland
Jun 19, 2012·Ageing Research Reviews·Verónica Gonzalez Pardo, Ana Russo de Boland
Jul 5, 2003·Physiological Reviews·Ralf M LoselMartin Wehling

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