Aged mice have increased inflammatory monocyte concentration and altered expression of cell-surface functional receptors.

Journal of Biosciences
Kelley StrohackerBrian K McFarlin

Abstract

The expression of monocyte cell-surface receptors represents one index of immune dysfunction, which is common with aging. Although mouse models of aging are prevalent, monocyte subset assessment is rare. Our purpose was to compare cell receptor expression on classic (CD115+/Gr-1 high) and non-classic (CD115+/Gr-1 low) monocytes from 80- or 20-week-old CD-1 mice. Three-colour flow cytometry was used to determine the concentration of monocyte subsets and their respective cell-surface expression of TLR2, TLR4, CD80, CD86, MHC II and CD54. These receptors were selected because they have been previously associated with altered monocyte function. Data were analysed with independent t-tests; significance was set at P less than 0.05. Old mice had a greater concentration of both classic (258%, P=0.003) and non-classic (70%, P=0.026) monocytes. The classic : non-classic monocyte ratio doubled in old as compared with that in young mice (P=0.006), indicating a pro-inflammatory shift. TLR4 ( 27%, P=0.001) and CD80 ( 37%, P=0.004) were decreased on classic monocytes from old as compared with those from young mice. TLR2 ( 24%, P=0.002) and MHCII ( 21%, P=0.026) were altered on non-classic monocytes from old as compared with those from young m...Continue Reading

References

Jun 6, 1998·Experimental Gerontology·N BoucherF Schächter
Feb 15, 2000·Experimental Gerontology·H M SadeghiJ L Fahey
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T MatsuguchiY Yoshikai
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mary RenshawSuryaprakash Sambhara
Oct 31, 2002·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·T StroffoliniA Craxi
Aug 29, 2003·The American Journal of Cardiology·Matteo CesariMarco Pahor
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cord SunderkötterPieter J M Leenen
Oct 30, 2004·Medicine and Science in Sports and Exercise·Brian K McFarlinKyle L Timmerman
May 21, 2005·Immunity & Ageing : I & a·Federico LicastroCalogero Caruso
Jun 21, 2005·Brain, Behavior, and Immunity·Laura K StewartErin Talbert
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Apr 14, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Brian K McFarlinPaul M Coen
Apr 14, 2006·Arquivos brasileiros de cardiologia·Abrahão Afiune NetoJosé Antonio F Ramires
Jan 4, 2007·The Journal of Clinical Investigation·Frank TackeGwendalyn J Randolph
Jan 4, 2007·The Journal of Clinical Investigation·Filip K SwirskiMikael J Pittet
Sep 22, 2007·Journal of Leukocyte Biology·Jean M DaleyJorge E Albina
Apr 7, 2011·Journal of Immunological Methods·W L BreslinB K McFarlin

❮ Previous
Next ❯

Citations

Feb 12, 2014·Experimental Gerontology·Niharika Arora DuggalJanet M Lord
May 9, 2014·Frontiers in Endocrinology·Sylvie RemaudBarbara A Demeneix
Dec 22, 2016·European Journal of Clinical Nutrition·B K McFarlinA S Venable
May 23, 2013·Endocrine Reviews·J BowersB A Demeneix
Jul 6, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jason TallisFrank Seebacher
Apr 13, 2021·International Journal of Food Sciences and Nutrition·Åsa HåkanssonBengt Jeppsson
Jun 1, 2021·Frontiers in Immunology·Miguel Ángel Palacios-PedreroGiulietta Saletti
Oct 29, 2021·Glia·Lindsey H Forbes, Veronique E Miron
Nov 7, 2021·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Cláudia Serre-MirandaMargarida Correia-Neves

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.