Aged Mouse Cortical Microglia Display an Activation Profile Suggesting Immunotolerogenic Functions

International Journal of Molecular Sciences
Tanja ZöllerBjörn Spittau

Abstract

Microglia are the resident immune cells of the central nervous system (CNS) and participate in physiological and pathological processes. Their unique developmental nature suggests age-dependent structural and functional impairments that might contribute to neurodegenerative diseases. In the present study, we addressed the age-dependent changes in cortical microglia gene expression patterns and the expression of M1- and M2-like activation markers. Iba1 immunohistochemistry, isolation of cortical microglia followed by fluorescence-activated cell sorting and RNA isolation to analyze transcriptional changes in aged cortical microglia was performed. We provide evidence that aging is associated with decreased numbers of cortical microglia and the establishment of a distinct microglia activation profile including upregulation of Ifi204, Lilrb4, Arhgap, Oas1a, Cd244 and Ildr2. Moreover, flow cytometry revealed that aged cortical microglia express increased levels of Cd206 and Cd36. The data presented in the current study indicate that aged mouse cortical microglia adopt a distinct activation profile, which suggests immunosuppressive and immuno-tolerogenic functions.

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Citations

Jun 10, 2020·The Journal of Pharmacology and Experimental Therapeutics·Maricedes Acosta-Martinez
May 13, 2018·Acta Neuropathologica·Rodney M RitzelLouise D McCullough
Oct 10, 2018·Frontiers in Aging Neuroscience·Barbara KleinLudwig Aigner
Dec 13, 2018·Experimental Biology and Medicine·Jessica Hersh, Shao-Hua Yang
Apr 17, 2020·Frontiers in Cellular Neuroscience·Alexander von EhrBjörn Spittau
Jul 18, 2018·International Journal of Molecular Sciences·Vladimir I Titorenko
Aug 22, 2018·Frontiers in Aging Neuroscience·Elena D OsipovaAlla B Salmina
May 28, 2020·International Journal of Molecular Sciences·Raffaella BoggiaAnna Pittaluga
Feb 2, 2019·International Journal of Molecular Sciences·Maria Olmedillas Del MoralOlga Garaschuk
Feb 2, 2021·Journal of Parkinson's Disease·Zijuan ZhangChristian Hölscher
May 15, 2020·Neurobiology of Aging·Chunxu YuanHong Qing
May 1, 2021·International Journal of Molecular Sciences·Mohammed E ChoudhuryJunya Tanaka
May 1, 2021·Antibody Therapeutics·Mi DengCheng Cheng Zhang
May 26, 2021·Journal of Pharmacological Sciences·Charlotte Isabelle DelageMarie-Ève Tremblay
Jul 3, 2021·Cells·Soyoung ChoiMaria Francesca Cordeiro
Aug 8, 2021·International Journal of Molecular Sciences·Mohammed E ChoudhuryJunya Tanaka

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Methods Mentioned

BETA
transgenic
fluorescence activated cell sorting
GTPase
FCS
chips
PCR
in vitro transcription

Software Mentioned

LAS AF
GraphPad Prism
DAVID
ImageJ
Stereoinvestigator

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