PMID: 6165871Apr 1, 1981Paper

Ageing studies in rat liver. II. Patterns of cellular and cell-free protein synthesis from one to ten months of age

Mechanisms of Ageing and Development
R R StoneR B Trimble

Abstract

Potential changes in liver protein biosynthesis were investigated in 1 to 10-month-old male Fischer F344 rats by isolation and translation of polysome-derived poly(A)-containing RNA and by incubation of isolated hepatocytes in Swim's S77 medium supplemented with [3H]leucine. Labeled protein products were analyzed by one- and two-dimensional polyacrylamide gel electrophoresis followed by fluorography. The protein profiles revealed that the translation products of poly(A)-containing RNA derived from the livers of 1 -months-old animals lacked a major 22000 dalton band which was present in comparable profiles from livers of older animals. In parallel experiments, gel profiles of products from cultured hepatocytes isolated from 1-month-old animals were missing an 18000 dalton band which was seen in preparations from older animals. With livers from rats between 2 and 10 months of age, however, no age-related changes in the pattern of protein biosynthesis were detected either in the products of poly(A)-containing RNA translated in vitro or in the cellular and secreted proteins of intact hepatocytes. Furthermore, the polysome content (A1(260)cm/g tissue) was constant between 1 and 10 months of age, providing a constant amount of tissue...Continue Reading

References

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Citations

Aug 1, 1983·Biological Reviews of the Cambridge Philosophical Society·S C Makrides
Nov 1, 1989·Journal of Cellular Physiology·J A ButlerA Richardson
Jun 8, 2018·Microbiology·Danielle V MillerRobert H White

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