Ager-CreERT2 : A New Genetic Tool for Studying Lung Alveolar Development, Homeostasis, and Repair

American Journal of Respiratory Cell and Molecular Biology
Mei-I Chung, Brigid L M Hogan

Abstract

The alveolar region of the lung is composed of two major epithelial cell types: cuboidal alveolar type 2 cells (AT2 cells), which produce surfactant proteins, and large, thin, alveolar type 1 cells (AT1 cells), specialized for efficient gas exchange. AT1 cells cover more than 95% of the alveolar surface and constitute a major barrier to the entry of pathogenic agents. Relatively few genetic tools are available for studying the development of AT1 cells, the function of genes expressed in them, and the effect of specifically killing them in vivo in the adult lung. One distinguishing feature of AT1 cells is the high level of expression of the gene Ager, encoding the advanced glycation endproduct-specific receptor, a member of the immunoglobulin superfamily of cell surface receptors. In this paper, we report the generation of a novel Ager-CreERT2 allele in which Cre recombinase is inserted into the first coding exon of the endogenous gene. After treatment with tamoxifen the allele enables Ager+ progenitor cells to be efficiently lineage labeled during late embryonic development and AT1 cells to be killed in the adult lung using a Rosa26-diphtheria toxin A allele. Significantly, adult mice in which approximately 50% of the AT1 cells...Continue Reading

References

Feb 1, 1975·Experimental and Molecular Pathology·M J EvansG Freeman
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·K C StoneJ D Crapo
Feb 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Renli QiaoZea Borok
Sep 1, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Angelika BierhausPeter P Nawroth
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·David VoehringerRichard M Locksley
Aug 8, 2013·The Journal of Clinical Investigation·Christina E BarkauskasBrigid L M Hogan
Feb 28, 2015·American Journal of Respiratory and Critical Care Medicine·Ewald R Weibel
Apr 14, 2015·Nature Communications·Rajan JainJonathan A Epstein
Nov 21, 2015·Development·Jun YangJichao Chen
Feb 9, 2018·Science·Ahmad N NabhanTushar J Desai
Feb 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yanjie WangNan Tang

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Citations

Dec 7, 2018·The Journal of Pathology·Svenja LoeringPhilip M Hansbro
Oct 18, 2019·The European Respiratory Journal·Soula DanopoulosDenise Al Alam
Jul 25, 2018·American Journal of Respiratory Cell and Molecular Biology·Carolina Garcia-de-AlbaCarla F Kim
Jul 15, 2020·Nature Cell Biology·Yoshihiko KobayashiPurushothama Rao Tata
Oct 10, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ettore LignelliRory E Morty
Nov 21, 2020·PLoS Biology·Alexandros SountoulidisChristos Samakovlis
Jan 20, 2021·Development·Huijuan Wu, Nan Tang
Sep 19, 2019·Biochimica Et Biophysica Acta. General Subjects·Babett BartlingAndreas Simm
Oct 20, 2020·Annual Review of Physiology·Konstantinos-Dionysios AlysandratosDarrell N Kotton
Jan 6, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Derek J Royer, Donald N Cook

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