AGEs and glucose levels modulate type I and III procollagen mRNA synthesis in dermal fibroblasts cells culture.

Experimental Diabetes Research
Serban Iren AndreeaDinischiotu Anca

Abstract

In the dermis, fibroblasts play an important role in the turnover of the dermal extracellular matrix. Collagen I and III, the most important dermal proteins of the extracellular matrix, are progressively altered during ageing and diabetes. For mimicking diabetic conditions, the cultured human dermal fibroblasts were incubated with increasing amounts of AGE-modified BSA and D-glucose for 24 hours. The expression of procollagen alpha2(I) and procollagen alpha1(III) mRNA was analyzed by quantitative real-time PCR. Our data revealed that the treatment of fibroblasts with AGE-modified BSA upregulated the expression of procollagen alpha2(I) and procollagen alpha1(III) mRNA in a dose-dependent manner. High glucose levels mildly induced a profibrogenic pattern, increasing the procollagen alpha2(I) mRNA expression whereas there was a downregulation tendency of procollagen alpha1(III) mRNA.

References

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Citations

Feb 15, 2011·Experimental Diabetes Research·Sandra M BarbalhoVanessa Sellis da Silva
May 12, 2012·Experimental Diabetes Research·Sheng-Neng XueLi Yan
May 18, 2011·BMC Cell Biology·Chi-Young WangHung-I Yeh
Dec 7, 2013·Chemico-biological Interactions·Subramanian UmadeviVellaichamy Elangovan
Feb 5, 2013·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Tao-Hua LanHong-Mei Tan
Apr 6, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Sihem BerdjaSouhila Aouichat Bouguerra
May 25, 2011·Journal of Cellular Biochemistry·Susana C RosaAlexandrina F Mendes
Feb 11, 2015·Journal of Periodontology·D Roselyn CerutisTakanari Miyamoto
Apr 30, 2016·Connective Tissue Research·Thomas J KeanJames E Dennis
Oct 21, 2017·The Journal of Biological Chemistry·Seunghwan SonJe-Wook Yu
Oct 23, 2018·Medicina·Marlon E Cerf
Mar 10, 2018·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Ae-Rang HwangYoung Jin Kang
Sep 13, 2014·World Journal of Emergency Medicine·Sheng-Neng XueLi Yan
Oct 9, 2020·European Journal of Medicinal Chemistry·Aurelia Magdalena PisoschiAndreea Iren Serban
Mar 7, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guang-Huar YoungHan-Min Chen
Jun 6, 2015·Physiological Research·M KnaśM Niczyporuk

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Methods Mentioned

BETA
glycosylation
fluorescence spectroscopy
electrophoresis
biopsy
PCR
antisense oligonucleotide
gel filtration

Software Mentioned

iCycler iQ TM
Beacon Designer

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