PMID: 8963718May 1, 1996Paper

Agglutination of isolated platelet membranes

Arteriosclerosis, Thrombosis, and Vascular Biology
Meinrad GawazF Neumann

Abstract

Platelet membrane glycoproteins play a central role in platelet aggregation and thus in primary hemostasis. To investigate mechanisms of platelet-platelet interaction in the absence of cellular activation events, we studied immunological and functional aspects of isolated platelet membranes. Platelet membranes contained significant amounts of the inducible fibrinogen receptor, glycoprotein (GP) IIb-IIIa, which exposes conformation-dependent LIBS1 and PMI-1 epitopes in response to fibrinogen-mimetic peptides GRGDSP and HHLG-GAKQAGDV. In the presence of soluble fibrinogen, membrane-coated latex beads showed Ca(2+)-dependent agglutination that could be partially inhibited by GRGDSP but not by the biologically inactive peptide GRGESP. Thrombospondin enhanced agglutination of membrane-coated beads, which could be inhibited by polyvalent anti-thrombospondin Fab fragments and anti-thrombospondin monoclonal antibody MA-II. Mg2+ inhibited both GPIIb-IIIa- and thrombospondin-mediated agglutination of membranes in a dose-dependent manner. The results of the present study indicate that isolated platelet membranes are a useful tool to study regulation of GPIIb-IIIa- and thrombospondin-mediated platelet-platelet interaction.

References

Aug 1, 1991·Kidney International·M P Gawaz, R A Ward
Feb 1, 1989·Archives of Biochemistry and Biophysics·S Jackowski, C O Rock
Jan 1, 1986·Annual Review of Medicine·L Leung, R Nachman
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K V WatsonH S Jacob
Jul 1, 1985·Thrombosis Research·W J BoothM C Berndt
Oct 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·R G HerrmannV G Crowe
Nov 1, 1984·The Journal of Clinical Investigation·L L Leung
Jul 1, 1994·Journal of the American Society of Nephrology : JASN·M P GawazS K Mujais
Dec 15, 1993·Thrombosis Research·S SatohN Sakuragawa

❮ Previous
Next ❯

Citations

Apr 16, 2003·Kidney International. Supplement·Stéphanie ViccaBernard Lacour
Feb 24, 2007·Vascular Health and Risk Management·James M BackesPatricia A Howard
Mar 18, 2017·International Journal of Cardiology·Marcin MakowskiZbigniew Baj
Apr 25, 2008·Platelets·Wei YinEllinor I B Peerschke

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

Related Papers

Biochemical and Biophysical Research Communications
Gerald M O'NeillAthar H Chishti
Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
T D Mountokalakis
© 2021 Meta ULC. All rights reserved