Aggrecan-like biomimetic proteoglycans (BPGs) composed of natural chondroitin sulfate bristles grafted onto a poly(acrylic acid) core for molecular engineering of the extracellular matrix

Acta Biomaterialia
Katsiaryna PrudnikovaMichele S Marcolongo

Abstract

Biomimetic proteoglycans (BPGs) were designed to mimic the three-dimensional (3D) bottlebrush architecture of natural extracellular matrix (ECM) proteoglycans, such as aggrecan. BPGs were synthesized by grafting native chondroitin sulfate bristles onto a synthetic poly(acrylic acid) core to form BPGs at a molecular weight of approximately ∼1.6 MDa. The aggrecan mimics were characterized chemically, physically, and structurally, confirming the 3D bottlebrush architecture as well as a level of water uptake, which is greater than that of the natural proteoglycan, aggrecan. Aggrecan mimics were cytocompatible at physiological concentrations. Fluorescently labeled BPGs were injected into the nucleus pulposus of the intervertebral disc ex vivo and were retained in tissue before and after static loading and equilibrium conditioning. BPGs infiltrated the tissue, distributed and integrated with the ECM on a molecular scale, in the absence of a bolus, thus demonstrating a new molecular approach to tissue repair: molecular matrix engineering. Molecular matrix engineering may compliment or offer an acellular alternative to current regenerative medicine strategies. Aggrecan is a natural biomolecule that is essential for connective tissue hy...Continue Reading

Citations

Nov 28, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Evan R PhillipsMichele S Marcolongo
May 6, 2019·Journal of Biomedical Materials Research. Part a·Evan R PhillipsMichele S Marcolongo
Feb 21, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Alicia S KrieteMichele S Marcolongo
Aug 28, 2020·Macromolecular Rapid Communications·Kerui ChenKai Guo
Feb 23, 2020·Frontiers in Pharmacology·Tanaya Walimbe, Alyssa Panitch

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