PMID: 9536289Apr 16, 1998Paper

Aggrecanase and metalloproteinase-specific aggrecan neo-epitopes are induced in the articular cartilage of mice with collagen II-induced arthritis

Osteoarthritis and Cartilage
I I SingerD M Visco

Abstract

To analyze the roles of two classes of proteinases, 'aggrecanase', and matrix metalloproteinases (MMPs), in chondrodestruction during murine collagen-induced arthritis (CIA). Generation of the 'aggrecanase' neo-epitope (NITEGE373), and the MMP neo-epitope (VDIPEN341) within aggrecan was studied by immunoperoxidase microscopy using specific anti-peptide antibodies in normal and stromelysin-1 (SLN-1) deficient knockout mice with CIA. High levels of NITEGE373 and VDIPEN341 neo-epitopes were observed in foci within CIA paw articular cartilage exhibiting depletion of glycosaminoglycans, in advance of significant cartilage erosion. The highest concentrations of NITEGE373 and VDIPEN341 labeling were observed and often co-distributed in the chondrocyte pericellular matrix, suggesting that stimulated chondrocytes can synthesize and/or activate both enzymes. Other regions of the cartilage frequently exhibited either NITEGE373 or VDIPEN341 labeling, but not both neo-epitopes simultaneously, suggesting that 'aggrecanase' and MMP cleavages of aggrecan may be generated independently. No detectable differences were observed in expression or distribution of either neo-epitope in SLN-1 knockout versus wild-type mice. In addition, in vitro diges...Continue Reading

References

Aug 1, 1989·The Journal of Clinical Investigation·D D DeanJ F Woessner
Jan 1, 1988·Methods in Enzymology·P H Wooley
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Nov 22, 1995·Biochemical and Biophysical Research Communications·H YamadaY Yamaguchi
Sep 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C O HanemannH W Müller
Apr 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A TonaD Dahl
May 3, 1996·The Journal of Biological Chemistry·A A ColeK E Kuettner

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Citations

Mar 29, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ann L ArmstrongMichael G Ehrlich
May 16, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Christopher B LittleBruce Caterson
Mar 31, 2005·Molecular and Cellular Biology·Christopher B LittleAmanda J Fosang
Jul 11, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Stephen P HenryBenoit de Crombrugghe
Jan 31, 2003·Expert Opinion on Therapeutic Targets·Ian M Clark, Andrew E Parker
Jun 5, 2004·Trends in Molecular Medicine·Ahuva NissimYuti Chernajovsky
Oct 7, 2003·Nature Biotechnology·Gill AdamsYuti Chernajovsky
Apr 26, 2005·The Journal of Biological Chemistry·Christopher J PoonAmanda J Fosang
May 26, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Farshid GuilakIngrid Meulenbelt

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