Aggrecanolysis and in vitro matrix degradation in the immature bovine meniscus: mechanisms and functional implications.

Arthritis Research & Therapy
Christopher G WilsonMarc E Levenston

Abstract

Little is known about endogenous or cytokine-stimulated aggrecan catabolism in the meniscal fibrocartilage of the knee. The objectives of this study were to characterize the structure, distribution, and processing of aggrecan in menisci from immature bovines, and to identify mechanisms of extracellular matrix degradation that lead to changes in the mechanical properties of meniscal fibrocartilage. Aggrecanase activity in the native immature bovine meniscus was examined by immunolocalization of the aggrecan NITEGE neoepitope. To investigate mechanisms of cytokine-induced aggrecan catabolism in this tissue, explants were treated with interleukin-1alpha (IL-1) in the absence or presence of selective or broad spectrum metalloproteinase inhibitors. The sulfated glycosaminoglycan (sGAG) and collagen contents of explants and culture media were quantified by biochemical methods, and aggrecan catabolism was examined by Western analysis of aggrecan fragments. The mechanical properties of explants were determined by dynamic compression and shear tests. The aggrecanase-generated NITEGE neoepitope was preferentially localized in the middle and outer regions of freshly isolated immature bovine menisci, where sGAG density was lowest and blood...Continue Reading

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Citations

Jan 24, 2014·Journal of Materials Science. Materials in Medicine·Albana Ndreu HaliliVasif Hasirci
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Jan 18, 2017·Connective Tissue Research·James F Nishimuta, Marc E Levenston
Jun 16, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Daniel J OhBrian D Snyder
Nov 12, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Eva G Baylon, Marc E Levenston
Dec 18, 2019·Connective Tissue Research·Solaiman TarafderChang H Lee
Apr 18, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Marina DanalacheUlf K Hofmann
Jun 15, 2020·Osteoarthritis and Cartilage·E G BaylonM E Levenston

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

MINITAB

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