Aggregate mesenchymal stem cell delivery ameliorates the regenerative niche for muscle repair

Journal of Tissue Engineering and Regenerative Medicine
Marissa A RuehleJarrod A Call

Abstract

Duchenne muscular dystrophy is a severe muscle wasting disease due to the absence of the dystrophin protein from the muscle cell membrane, which renders the muscle susceptible to continuous damage. In Duchenne muscular dystrophy patients, muscle weakness, together with cycles of degeneration/regeneration and replacement with noncontractile tissue, limit mobility and lifespan. Because the loss of dystrophin results in loss of polarity and a reduction in the number of self-renewing satellite cells, it is postulated that these patients could achieve an improved quality of life if delivered cells could restore satellite cell function. In this study, we used both an established myotoxic injury model in wild-type (WT) mice and mdx mice alone (spontaneous muscle damage). Single (SC) and aggregated (AGG) mesenchymal stem cells (MSCs) were injected into the gastrocnemius muscles 4 hr after injury (WT mice). The recovery of peak isometric torque was longitudinally assessed over 5 weeks, with earlier takedowns for histological assessment of healing (fibre cross-section area and central nucleation) and MSC retention. AGG-treated WT mice had significantly greater torque recovery at Day 14 than SC or saline-treated mice and a greater CSA at ...Continue Reading

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Citations

Jan 17, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Marissa A RuehleNick J Willett
Jul 16, 2019·Journal of Tissue Engineering and Regenerative Medicine·Muhamed TalovicKoyal Garg
Feb 27, 2020·Biotechnology and Bioengineering·Gilad DoronJohnna S Temenoff
Jul 7, 2020·Tissue Engineering. Part a·Molly E OgleJohnna S Temenoff
Nov 26, 2020·Scientific Reports·Brent M BijonowskiSamuel C Grant
Sep 23, 2021·Journal of Tissue Engineering and Regenerative Medicine·Peter GenoveseKoyal Garg

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