Aggregated form of dextransucrases from Leuconostoc mesenteroides NRRL B-512F and its constitutive mutant

Bioscience, Biotechnology, and Biochemistry
K FunaneM Kobayashi

Abstract

Purified dextransucrases [EC 2.4.1.5], DSW-D and DSW-G, from Leuconostoc mesenteroides B-512F were obtained from affinity chromatography with DEAE-Sephadex A-50 by elution with clinical dextran and guanidine-HCl, respectively. DSM-G was purified from the B-512F mutant strain SH 3002, which produces dextransucrase constitutively. Although the sugar contents of the purified enzymes were different, their molecular masses by SDS-PAGE were all 170 kDa. DSW-D and DSW-G were highly aggregated and the all the activities were eluted at the void volume (V0) on Sepharose 6B, while the DSM-G was eluted at 1.2 x V0 volume. On rechromatography, DSM-G was separated into three peaks corresponding to the aggregated form, monomeric form, and partially digested form, respectively. The aggregation of Leuconostoc dextransucrase was looser than that of streptococcal glucosyltransferases, but the structures of these enzymes had high homology with each other.

Citations

Sep 1, 2007·Indian Journal of Microbiology·Avishek MajumderArun Goyal
Mar 22, 2007·Journal of Enzyme Inhibition and Medicinal Chemistry·Arun GoyalSarvagya S Katiyar
Mar 21, 1998·Bioscience, Biotechnology, and Biochemistry·K FunaneM Kobayashi
Dec 3, 1999·Applied and Environmental Microbiology·M QuirascoA Farrés
Oct 17, 2008·Carbohydrate Research·John F RobytRupendra Mukerjea

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