Aggregated low-density lipoprotein uptake induces membrane tissue factor procoagulant activity and microparticle release in human vascular smooth muscle cells

Circulation
V Llorente-CortésL Badimon

Abstract

Tissue factor (TF) is the main initiator of the arterial blood coagulation system, and aggregated LDL (agLDL) are found in the arterial intima. Our hypothesis is that agLDL internalization by vascular smooth muscle cells (VSMCs) may trigger TF-procoagulant activity. Cultured human VSMCs were obtained from human coronary arteries of explanted hearts during transplant operations. VSMCs were incubated with native LDL (nLDL) or agLDL. TF mRNA was analyzed by real-time polymerase chain reaction, and cellular and released TF protein antigen were analyzed by Western blot. TF microparticle (MP) content was analyzed by flow cytometry and TF activity by a factor Xa generation test. Both nLDL and agLDL strongly and equally increased TF mRNA and cell membrane protein expression, by approximately 5- and 9-fold, respectively. A sustained TF procoagulant activity was induced by agLDL but not by nLDL (agLDL 2.46+/-0.22 versus nLDL 0.72+/-0.12 mU/mg protein at 12 hours). AgLDL increased TF antigen release (agLDL 5.64+/-0.4 versus nLDL 3.28+/-0.22 AU) and TF MP release (agLDL 89.85+/-8.51 versus nLDL 19.69+/-4.59 TF MP/10(3) cells). TF activation and release induced by agLDL is not related to apoptosis. Blockade of LDL receptor-related protein, ...Continue Reading

References

Nov 14, 1997·The Journal of Biological Chemistry·A D SchecterM B Taubman
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·P L GiesenY Nemerson
Oct 25, 2001·Blood·D ArdissinoP M Mannucci
Jun 19, 2002·Trends in Cardiovascular Medicine·Peter van der Geer
Nov 12, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Vicenta Llorente-CortésLina Badimon
Mar 26, 2003·Nature Medicine·Vladimir Y BogdanovYale Nemerson
Aug 12, 2003·Cardiovascular Research·Marja J VanWijkR Nieuwland

❮ Previous
Next ❯

Citations

Aug 26, 2011·Seminars in Immunopathology·Olivier MorelFlorence Toti
Jan 1, 2010·Indian Journal of Clinical Biochemistry : IJCB·Kavita K ShaliaP K Thakur
Jan 7, 2010·Current Opinion in Nephrology and Hypertension·Chantal M Boulanger
Apr 12, 2011·Thrombosis and Haemostasis·Lina BadimonGemma Vilahur
Apr 23, 2015·Journal of Cellular Physiology·Priscilla GrossIsabelle Six
Jun 19, 2013·Clínica e investigación en arteriosclerosis : publicación oficial de la Sociedad Española de Arteriosclerosis·David de Gonzalo-CalvoVicenta Llorente-Cortés
Sep 16, 2009·Free Radical Biology & Medicine·Jane A Leopold, Joseph Loscalzo
Jun 15, 2007·Journal of Thrombosis and Haemostasis : JTH·D M Monroe, N S Key
Jun 10, 2010·Journal of Cellular Physiology·Véronique J MoulinHervé Genest
May 3, 2012·Annals of the New York Academy of Sciences·Lina Badimon, Gemma Vilahur
Oct 13, 2009·Journal of Thrombosis and Haemostasis : JTH·S Camino-LópezV Llorente-Cortés
Aug 27, 2014·Journal of Internal Medicine·L Badimon, G Vilahur
Oct 23, 2009·Clinical Science·Alexander BreitensteinFelix C Tanner
Apr 23, 2014·Free Radical Biology & Medicine·Michael Craig LarsonNeil Hogg
Sep 15, 2014·The International Journal of Biochemistry & Cell Biology·Valerie SamouillanVicenta Llorente-Cortés
Feb 13, 2016·The Journal of Physiology·A N Kapustin, C M Shanahan
Jul 2, 2016·Clínica e investigación en arteriosclerosis : publicación oficial de la Sociedad Española de Arteriosclerosis·David de Gonzalo-CalvoVicenta Llorente-Cortes
Jan 7, 2009·Therapeutic Advances in Cardiovascular Disease·Matteo PirroMassimo R Mannarino
Apr 1, 2012·European Heart Journal. Acute Cardiovascular Care·Lina BadimonGemma Vilahur
Dec 6, 2017·Therapeutic Advances in Hematology·Muriel Giansily-Blaizot, Jean-François Schved
Jan 13, 2018·Frontiers in Cardiovascular Medicine·Lina BadimonTeresa Padró
Aug 20, 2011·Circulation Research·Pierre-Emmanuel RautouChantal M Boulanger
Oct 29, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Vicenta Llorente-CortésLina Badimon
Sep 23, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Olivier MorelJean-Marie Freyssinet
Feb 12, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Vicenta Llorente-Cortés, Lina Badimon
May 2, 2015·Stroke; a Journal of Cerebral Circulation·Licia IacovielloMaria Benedetta Donati
Dec 25, 2009·Circulation. Cardiovascular Genetics·Manuel MayrChantal M Boulanger
Apr 22, 2018·Journal of Lipid Research·Frank W Pfrieger, Nicolas Vitale
Apr 1, 2005·Journal of Microencapsulation
Apr 23, 2019·Annals of Medicine·Christos VoukalisGregory Y H Lip

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiology Journals

Discover the latest cardiology research in this collection of the top cardiology journals.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis