Aggregatibacter actinomycetemcomitans H-NS promotes biofilm formation and alters protein dynamics of other species within a polymicrobial oral biofilm

NPJ Biofilms and Microbiomes
K BaoJan Oscarsson

Abstract

Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid-structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, and substantially lower monospecies biofilm formation relative to the wild type. Furthermore, the D7S hns mutant exhibited significantly reduced growth within a seven-species oral biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species regardless of being co-cultivated with D7S hns or its parental strain. Proteomics analysis of the strains grown in monocultures confirmed the role of H-NS as a repressor of gene expression in A. actinomycetemcomitans. Interestingly, proteomics analysis of the multispecies biofilms indicated that the A. actinomycetemcomitans wi...Continue Reading

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Citations

Jul 30, 2019·Journal of Dental Research·D P MillerR J Lamont
Nov 9, 2019·Pathogens·Georgios N BelibasakisAnders Johansson
Nov 24, 2020·Periodontology 2000·Nagihan BostanciGeorgios N Belibasakis

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Datasets Mentioned

BETA
CP003496
AFI86019
PXD008444

Methods Mentioned

BETA
atomic force microscopy
AFM
PCR
electrophoresis
Protein Assay
acetylation

Software Mentioned

ProgenesisQI
Scaffold
GraphPad
quantable
REVIGO
R language
mascot
gplots
Progenesis
CRAN

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