Aggregatibacter actinomycetemcomitans Invasion Induces Interleukin-1β Production Through Reactive Oxygen Species and Cathepsin B

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
Toshinori OkinagaTatsuji Nishihara

Abstract

Interleukin-1 (IL-1) cytokines, IL-1α, IL-1β, and IL-18 play a crucial role in inflammatory responses in a variety of diseases including periodontitis. In this study, the periodontopathic bacterial pathogen, Aggregatibacter actinomycetemcomitans, induced cell death and cytokine release in macrophages. Cell viability was reduced by A. actinomycetemcomitans invasion using (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay. The production of IL-1β in A. actinomycetemcomitans-invaded macrophage cells was detected by real-time reverse transcriptase-polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assay. Treatment with a caspase-1 inhibitor and silencing of the caspase-1 gene had no effect on IL-1β secretion induced by A. actinomycetemcomitans invasion. Pattern recognition receptor, NLRP3 was upregulated in A. actinomycetemcomitans-invaded macrophages. However, NLRP3 knockdown had no effect on the secretion of IL-1β in A. actinomycetemcomitans-invaded RAW 264 cells. In addition, A. actinomycetemcomitans invasion induced the generation of reactive oxygen species (ROS) and the release of cathepsin B in RAW 264 cells. Interestingly, CA074-Me, a cathepsin B inhibitor, and N-Acetyl-l-cysteine...Continue Reading

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Citations

Apr 21, 2016·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Christopher WynickKatrina Gee
Mar 16, 2017·Journal of Cellular Biochemistry·Mai OtsukaTatsuji Nishihara
Jan 22, 2020·Journal of Periodontal Research·Kübra AralPaul R Cooper
Sep 24, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eun-Chong HanHeon-Jin Lee
Jun 20, 2020·Nanotoxicology·Robert A YokelUschi M Graham
Jul 25, 2019·Journal of Clinical Medicine·Jan OscarssonAnders Johansson
Nov 23, 2017·Frontiers in Physiology·Patricia Hernández-RíosMarcela Hernández
Jan 5, 2020·International Journal of Oral Science·Ran ChengTao Hu
Jul 7, 2018·Inflammation and Regeneration·Junya KanekoTatsuji Nishihara
Jun 29, 2021·Frontiers in Immunology·Yang LiQianzhou Jiang
Nov 2, 2021·Frontiers in Microbiology·Ok-Jin ParkSeung Hyun Han

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Methods Mentioned

BETA
protein assay
electrophoresis
PCR
Fluorescence
enzyme-linked immunosorbent assay
flow cytometry
ELISA

Software Mentioned

Primer Express
Image Lab
MultiCycle

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