PMID: 8592147Feb 1, 1996Paper

Aggregation and metal-binding properties of mutant forms of the amyloid A beta peptide of Alzheimer's disease

Journal of Neurochemistry
A ClementsC H Williams

Abstract

The fibrillogenic properties of Alzheimer's A beta peptides corresponding to residues 1-40 of the normal human sequence and to two mutant forms containing the replacement Ala21 to Gly or Glu22 to Gln were compared. At pH 7.4 and 37 degrees C the Gln22 peptide was found to aggregate and precipitate from solution faster than the normal A beta, whereas the Gly21 peptide aggregated much more slowly. Electron microscopy showed that the aggregates all had fibrillar structures. Circular dichroism spectra of these peptides revealed that aggregation of the normal and Gln22 sequences was associated with spectral changes consistent with a transformation from random coil to beta sheet, whereas the spectrum of the Gly21 peptide remained almost unchanged during a period in which little or no aggregation occurred. When immobilised by spotting onto nitrocellulose membranes the peptides bound similar amounts of the radioisotope 65Zn2+. Of several competing metal ions, tested at 20x the concentration of Zn2+, Cu2+ displaced > 95% of the radioactivity from all three peptides and Ni2+ produced >50% displacement in each case. Some other metal ions tested caused lesser displacement, but Fe2+ and Al3+ were without effect. In a saturation binding assa...Continue Reading

Citations

Dec 2, 2008·Journal of Cellular and Molecular Medicine·Yif'at BiranPaul A Adlard
Oct 24, 2009·Protein Engineering, Design & Selection : PEDS·Dave E DunstanJoseph Bertolini
Jun 18, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Joo-Yong LeeJae-Young Koh
Aug 29, 2014·Frontiers in Aging Neuroscience·Robert A Marr, Daniel M Hafez
Jun 18, 2015·Molecular Neurobiology·Matic PavlinJanez Mavri
Jun 21, 2017·Biophysics Reviews·Katie L Stewart, Sheena E Radford
Sep 25, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Elena Atrián-BlascoChristelle Hureau
Oct 23, 2019·Signal Transduction and Targeted Therapy·Pei-Pei LiuJian-Sheng Kang
Mar 1, 1997·Protein Science : a Publication of the Protein Society·T G Fletcher, D A Keire
May 19, 2004·The European Journal of Neuroscience·Igor KlyubinMichael J Rowan
May 25, 2005·International Journal of Experimental Pathology·Christa J MaynardQiao-Xin Li
Feb 22, 2008·Journal of Neurochemistry·Vello TõuguPeep Palumaa
Aug 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Giuseppe Arena, Enrico Rizzarelli
May 8, 2020·The Journal of Biological Chemistry·Elliot J CrooksLisa M Miller
Jul 6, 2002·Anais Da Academia Brasileira De Ciências·Fernanda G Defelice, Sergio T Ferreira
Aug 4, 2005·Chembiochem : a European Journal of Chemical Biology·Yasmina MekmouchePeter Faller
May 19, 2010·Journal of Molecular Neuroscience : MN·Nagarajan SureshbabuR Jayakumar
Aug 28, 1999·The Journal of Biological Chemistry·D M WalshD B Teplow
Mar 27, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Peter Faller, Christelle Hureau
Oct 31, 2012·International Journal of Molecular Sciences·Fujun YaoXiangjun Li
Nov 24, 2017·International Journal of Molecular Sciences·Stuart D Portbury, Paul A Adlard
Aug 29, 1997·The Journal of Biological Chemistry·D M WalshD B Teplow
Aug 1, 1998·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·D B Teplow
Jan 16, 2007·The FEBS Journal·Jens DanielssonAstrid Gräslund
Mar 13, 2009·Journal of Cellular and Molecular Medicine·Katherine A PriceCyril C Curtain
Apr 5, 2020·Journal of Clinical Medicine·Sang Hoon YoonJeong Tae Do
Jun 26, 2001·European Journal of Biochemistry·M BrzyskaD Elbaum
Jul 4, 2003·The Journal of Biological Chemistry·Gal BitanDavid B Teplow
Feb 1, 2021·The Journal of Biological Chemistry·Elliot J CrooksLisa M Miller
Aug 23, 2020·Signal Transduction and Targeted Therapy·Pei-Pei LiuJian-Sheng Kang
Jan 1, 1997·Neurobiology of Disease·M P Cuajungco, G J Lees
Aug 28, 1999·Bioorganic & Medicinal Chemistry Letters·W Garzon-RodriguezC G Glabe
Apr 1, 1997·Brain Research. Brain Research Reviews·M P Cuajungco, G J Lees

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