PMID: 1534998Jun 16, 1992

Aggregation of IgE-receptor complexes on rat basophilic leukemia cells does not change the intrinsic affinity but can alter the kinetics of the ligand-IgE interaction

Biochemistry
R G PosnerB Goldstein

Abstract

The aggregation of IgE anchored to high-affinity Fc epsilon receptors on rat basophilic leukemia (RBL) cells by multivalent antigens initiates transmembrane signaling and ultimately cellular degranulation. Previous studies have shown that the rate of dissociation of bivalent and multivalent DNP ligands from RBL cells sensitized with anti-DNP IgE decreases with increasing ligand incubation times. One mechanism proposed for this effect is that when IgE molecules are aggregated, a conformational change occurs that results in an increase in the intrinsic affinity of IgE for antigen. This possibility was tested by measuring the equilibrium constant for the binding of monovalent DNP-lysine to anti-DNP IgE under two conditions, where the cell-bound IgE is dispersed and where it has been aggregated into visible patches on the cell surface using anti-IgE and a secondary antibody. No difference in the equilibrium constant in these two cases was observed. We also measured the rate of dissociation of a monovalent ligand from cell surface IgE under these two conditions. Whereas the affinity for monovalent ligand is not altered by IgE aggregation, we observe that the rate of ligand dissociation from IgE in clusters is slower than the rate of...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R G AndersonM S Brown
Nov 1, 1977·Biophysical Journal·H C Berg, E M Purcell
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T L Hill
Jan 1, 1988·Science·T A RyanW W Webb
Jan 1, 1986·Annual Review of Immunology·Henry MetzgerR Quarto
Feb 1, 1982·Molecular Immunology·David Holowka, Henry Metzger
Nov 25, 1983·Journal of Immunological Methods·Barbara BairdS Mazlin

Citations

Jan 1, 1994·European Biophysics Journal : EBJ·A A PotaninF W Wiegel
Jan 1, 1997·Annals of Biomedical Engineering·M A Tsai, D A Hammer
Mar 23, 2013·Archives of Pharmacal Research·Marcela de Souza SantosRose Mary Zumstein Georgetto Naal
Dec 1, 1994·Molecular Immunology·R G Posner, M Dembo
Aug 4, 2012·Journal of Leukocyte Biology·Jinmin LeeDavid Holowka
Jun 25, 2009·Journal of Cell Science·Lavanya VasudevanBarbara Baird
Dec 7, 2007·Biosensors & Bioelectronics·Theresa CurtisDavid Holowka
Mar 11, 2008·Biophysical Journal·Raibatak DasBarbara Baird
Jul 15, 2009·British Journal of Pharmacology·A SzczukaGeorges Vauquelin
Oct 1, 2010·British Journal of Pharmacology·Georges Vauquelin, Steven J Charlton
Oct 26, 2012·Parasite Immunology·S M ThrasherJ A Appleton
Nov 4, 2004·Biosensors & Bioelectronics·Rose Mary Z G NaalBarbara Baird
Nov 22, 1997·Current Biology : CB·R N Germain
Feb 13, 2013·International Immunopharmacology·Marcela de Souza SantosRose Mary Zumstein Georgetto Naal
Sep 10, 2005·Biophysical Journal·Manoj GopalakrishnanUwe C Täuber
Apr 17, 2014·Molecular BioSystems·Mehdi Bouhaddou, Marc R Birtwistle
Jun 12, 2016·Traffic·Joshua D WilsonBarbara Baird
Nov 13, 2004·The Journal of Biological Chemistry·Ryan M YoungBarbara Baird
Oct 4, 2007·The Journal of Biological Chemistry·Michael L DustinAdrian Whitty
Oct 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roy CohenBarbara Baird
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie A GosseBarbara Baird
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jodi M PaarBarbara Baird
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·James R FaederByron Goldstein
Mar 7, 2019·Royal Society Open Science·Md ShahinuzzamanDipak Barua
Nov 5, 1999·The Journal of Biological Chemistry·R G DiScipioN K Rushmere
Dec 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valdemir C SilvaMarília O F Goulart

Related Concepts

Plasma Cell Antigens PC-1
Immunoglobulin E
Receptor Aggregation
Fc Receptor
Tumor Cells, Cultured
Acute Basophilic Leukemia
Low affinity IgE receptor

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