Aggregation of IgE receptors in rat basophilic leukemia 2H3 cells induces tyrosine phosphorylation of the cytosolic protein-tyrosine phosphatase HePTP.

The Journal of Biological Chemistry
M SwieterR P Siraganian

Abstract

The cDNA encoding the rat equivalent of the human hematopoietic tyrosine phosphatase, also known as leukocyte phosphatase, was isolated from a rat basophilic leukemia mast cell cDNA library. By two-dimensional electrophoresis, the protein expressed in the mast cells was of a size (40 kDa) and pI (6.9) predicted from the deduced amino acid sequence. Thus, although previously shown to be preferentially expressed in T cells and B cells, the phosphatase is also found in mast cells. By immunofluorescence microscopy, rat hematopoietic tyrosine phosphatase localized to discrete, globular compartments within the cytoplasm and was not found either in the nucleus or associated with the cell surface membrane. Aggregation of high affinity IgE receptors in the mast cells induced tyrosine phosphorylation of the phosphatase. The tyrosine phosphorylation was mimicked by stimulation with calcium ionophore A23187 but not by direct activation of protein kinase C. Since phosphorylation of the phosphatase was dramatically reduced when the cells were activated in Ca(2+)-free media, it is dependent on a rise in intracellular Ca2+. These data strongly suggest that hematopoietic tyrosine phosphatase may be involved in the IgE receptor-mediated signalin...Continue Reading

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Citations

May 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J A Frearson, D R Alexander
Jun 1, 1997·Current Opinion in Immunology·B G Neel
Jan 1, 1997·Annual Review of Immunology·M Daëron
Mar 18, 2009·Immunological Reviews·Alasdair M Gilfillan, Juan Rivera
May 15, 2007·Immunological Reviews·Jakub Abramson, Israel Pecht
Sep 21, 2000·Seminars in Immunology·M Saxena, T Mustelin
Jul 1, 2008·Experimental Cell Research·Adriana Maria Mariano Silveira e SouzaConstance Oliver
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Z H XieR P Siraganian

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