Abstract
It has been shown that the magnitude of DNA synthesis and the time at which maximal DNA synthesis occurs after two-thirds partial hepatectomy (PH) is greatly reduced in the liver of aged rodents compared to young animals. This reduction could represent an intrinsic defect in proliferation or a more specialized change in the response to PH. We therefore evaluated the proliferative capacity of hepatocytes in aged animals, following treatment with primary liver mitogens. We show that treatment of 12-month-old CD-1 mice with the hepatomitogen 1,4-bis[2-(3,5-dichloropyridyloxy)]benzene (TCPOBOP) caused an increase in hepatocyte proliferation similar to that seen in young (8-week-old) mice. The labeling index was 82% in the livers of aged mice versus 76% in young animals. Histological observation demonstrated that the number of hepatocytes entering mitoses was similar in both groups; the mitotic indices were 2.5 per thousand and 2.7 per thousand, respectively. Additional experiments showed that the timing of DNA synthesis and M phase were nearly identical in both aged and young mice. Stimulation of hepatocyte DNA synthesis was associated with increased expression of several cell cycle-associated proteins (cyclin D1, cyclin A, cyclin ...Continue Reading
Citations
Apr 21, 2010·PloS One·Yukio YamamotoMasahiko Negishi
Feb 8, 2014·PloS One·Birgit BerkenkampRoland Schmitt
Nov 21, 2007·Biological Chemistry·Inken Wierstra, Jürgen Alves
May 10, 2011·Rejuvenation Research·Meriem Mahrouf-YorgovHélène Gilgenkrantz
Apr 11, 2009·Trends in Endocrinology and Metabolism : TEM·Nikolai A Timchenko
Nov 25, 2006·Gene·Young-Kyo SeoBandana Chatterjee
Oct 27, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Robert H CostaI-Ching Wang
Apr 22, 2008·Cell Proliferation·A ColumbanoG M Ledda-Columbano
Sep 18, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·William E Blanco-BoseAndreas Trumpp
Jan 17, 2012·International Journal of Experimental Pathology·Edina BugyikPeter Nagy
Oct 4, 2006·Biochimica Et Biophysica Acta·Jamila LaoukiliRené H Medema
Oct 19, 2005·Gastroenterology·Karen E HallSuzanne Rose
Jun 4, 2015·Age·Monica PibiriMaria Ravo
Aug 11, 2011·Journal of Hepatology·Tenghui ChenChundong Yu
Jan 27, 2015·Journal of Hepatology·Maria Paola SerraEzio Laconi
Oct 22, 2013·The American Journal of Pathology·Jiansheng Huang, David A Rudnick